Immunoglobulin V-lambda transcription profiling of systemic lupus erythematosus patients reveals biased usage of genes located near the J lambda-C lambda segments

Autor(es)

Trevisan, G. L. ; Tamia-Ferreira, M. C. ; Passos, G. A. S. ; Junta, C. M. ^[4]

• Tipo de documento: Artigo Científico
• Fonte: Scandinavian Journal of Immunology; v. 59, n. 4, p. 395-399, abr. 2004.
• Área do conhecimento: Ciências Biológicas - Imunologia
• Assunto(s): Lúpus eritematoso cutâneo Expressão gênica Imunoglobulinas

Resumo

To evaluate the distribution of usage and to quantify the transcription levels of the immunoglobulin lambda variable (IGLV) genes in patients with systemic lupus erythematosus (SLE) and normal individuals (NIs), cDNA samples from peripheral blood lymphocytes were prepared and probed with IGLV-specific oligonucleotides. Because recombinations involving V-lambda pseudogenes are nonproductive, we analysed the IGLV productive repertoire, as cDNAs were copied from IGLV mRNA producing B lymphocytes. Increased expression of the IGLV8a gene in SLE led us to analyse the transcription levels of all IGLV genes. We developed an expression profiling approach to scan the entire V-lambda locus on chromosome 22q11.2. The transcription profiling showed that usually the V-lambda genes located near the J-C cluster were preferentially expressed in both groups, i.e. patients and NIs, with the expression levels of SLE patients being significantly higher. However, genes displaying peaks of expression independent of J-C cluster proximity were observed along the IGLV locus. Our data permit us to conclude that there are differences in V-lambda gene expression between SLE patients and NIs, and a preferential usage of genes located near the J-C cluster. The data also demonstrate the occurrence of V-J-C-productive recombinations independent of gene localization along the locus. (AU)

• Processo FAPESP: 98/05584-9 - Sequenciamento, recombinação e expressão de genes V-lambda das imunoglobulinas humanas no lúpus eritematoso sistêmico
• Beneficiário: Geraldo Aleixo da Silva Passos Júnior
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 98/09789-4 - Emergência da recombinação V(D)J nos locus TCRbeta e expressão gênica diferencial durante a ontogenia do timo
• Beneficiário: Geraldo Aleixo da Silva Passos Júnior
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 00/12495-4 - Extensão Projeto Genoma Câncer
• Beneficiário: Geraldo Aleixo da Silva Passos Júnior
• Modalidade de apoio: Auxílio à Pesquisa - Programa GENOMA
• Processo FAPESP: 99/12135-9 - Projeto transcriptoma: análise da expressão gênica em larga escala usando DNA-arrays
• Beneficiário: Geraldo Aleixo da Silva Passos Júnior
• Modalidade de apoio: Auxílio à Pesquisa - Temático