| Full text | |
| Author(s): |
Trevisan, G. L.
;
Tamia-Ferreira, M. C.
;
Passos, G. A. S.
;
Junta, C. M.
[4]
Total Authors: 4
|
| Document type: | Journal article |
| Source: | Scandinavian Journal of Immunology; v. 59, n. 4, p. 395-399, abr. 2004. |
| Field of knowledge: | Biological Sciences - Immunology |
| Abstract | |
To evaluate the distribution of usage and to quantify the transcription levels of the immunoglobulin lambda variable (IGLV) genes in patients with systemic lupus erythematosus (SLE) and normal individuals (NIs), cDNA samples from peripheral blood lymphocytes were prepared and probed with IGLV-specific oligonucleotides. Because recombinations involving V-lambda pseudogenes are nonproductive, we analysed the IGLV productive repertoire, as cDNAs were copied from IGLV mRNA producing B lymphocytes. Increased expression of the IGLV8a gene in SLE led us to analyse the transcription levels of all IGLV genes. We developed an expression profiling approach to scan the entire V-lambda locus on chromosome 22q11.2. The transcription profiling showed that usually the V-lambda genes located near the J-C cluster were preferentially expressed in both groups, i.e. patients and NIs, with the expression levels of SLE patients being significantly higher. However, genes displaying peaks of expression independent of J-C cluster proximity were observed along the IGLV locus. Our data permit us to conclude that there are differences in V-lambda gene expression between SLE patients and NIs, and a preferential usage of genes located near the J-C cluster. The data also demonstrate the occurrence of V-J-C-productive recombinations independent of gene localization along the locus. (AU) | |
| FAPESP's process: | 99/12135-9 - Transcriptome project: large scale gene expression analysis using DNA-Arrays |
| Grantee: | Geraldo Aleixo da Silva Passos Júnior |
| Support Opportunities: | Research Projects - Thematic Grants |