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Characterization of novel lncRNA muscle expression profiles associated with meat quality in beef cattle

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Autor(es):
Magalhaes Muniz, Maria Malane ; Simielli Fonseca, Larissa Fernanda ; Becker Scalez, Daiane Cristina ; Vega, Aroa Suarez ; dos Santos Silva, Danielly Beraldo ; Ferro, Jesus Aparecido ; Chardulo, Artur Loyola ; Baldi, Fernando ; Canovas, Angela ; de Albuquerque, Lucia Galvao
Número total de Autores: 10
Tipo de documento: Artigo Científico
Fonte: EVOLUTIONARY APPLICATIONS; v. 15, n. 4, p. 13-pg., 2022-03-25.
Resumo

The aim of this study was to identify novel lncRNA differentially expressed (DE) between divergent animals for beef tenderness and marbling traits in Nellore cattle. Longissimus thoracis muscle samples from the 20 most extreme bulls (of 80 bulls set) for tenderness, tender (n = 10) and tough (n = 10) groups, and marbling trait, high (n = 10) and low (n = 10) groups were used to perform transcriptomic analysis using RNA-Sequencing. For tenderness, 29 lncRNA were DE (p-value <= 0.01) in tough beef animals in relation to tender beef animals. We observed that genic lncRNAs, for example, lncRNA_595.1, were overlapping exonic part of the PICK gene, while lncRNA_3097.2 and lncRNA_3129.5 overlapped intronic part of the genes GADL1 and PSMD6. The lncRNA associated with PICK1, GADL1, and PMD6 genes were enriched in the pathways associated with the ionotropic glutamate receptor, gamma-aminobutyric acid synthesis, and the ubiquitin-proteasome pathway. For marbling, 50 lncRNA were DE (p-value <= 0.01) in high marbling group compared with low marbling animals. The genic lncRNAs, such as lncRNA_3191.1, were overlapped exonic part of the ITGAL gene, and the lncRNA_512.1, lncRNA_3721.1, and lncRNA_41.4 overlapped intronic parts of the KRAS and MASP1 genes. The KRAS and ITGAL genes were enriched in pathways associated with integrin signaling, which is involved in intracellular signals in response to the extracellular matrix, including cell form, mobility, and mediates progression through the cell cycle. In addition, the lincRNAs identified to marbling trait were associated with several genes related to calcium binding, muscle hypertrophy, skeletal muscle, lipase, and oxidative stress response pathways that seem to play a role important in the physiological processes related to meat quality. These findings bring new insights to better understand the biology mechanisms involved in the gene regulation of these traits, which will be valuable for a further investigation of the interactions between lncRNA and mRNAs, and of how these interactions may affect meat quality traits. (AU)

Processo FAPESP: 18/11154-2 - Identificação de genes de co-expressão em tecido muscular de bovinos Nelore divergente para fenótipos de marmoreio e índice de fragmentação miofibrilar, usando dados de RNA-Seq
Beneficiário:Maria Malane Magalhães Muniz
Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
Processo FAPESP: 18/20026-8 - EMU concedido no processo 2017/10630-2: servidor
Beneficiário:Lucia Galvão de Albuquerque
Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
Processo FAPESP: 09/16118-5 - Ferramentas genômicas no melhoramento genético de características de importância econômica direta em bovinos da raça Nelore
Beneficiário:Lucia Galvão de Albuquerque
Modalidade de apoio: Auxílio à Pesquisa - Temático
Processo FAPESP: 17/10630-2 - Aspectos genéticos da qualidade, eficiência e sustentabilidade da produção de carne em animais da raça Nelore
Beneficiário:Lucia Galvão de Albuquerque
Modalidade de apoio: Auxílio à Pesquisa - Temático
Processo FAPESP: 11/21241-0 - Estudo da variabilidade genética do perfil de ácidos graxos da carne de bovinos da raça Nelore terminados em confinamento
Beneficiário:Fernando Sebastián Baldi Rey
Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores