Longitudinal Evaluation of Ethyl Cyanoacrylate Adhesives on Candida albicans Biofilm

Autor(es)

Helena Sandrini Venante ^[1] ; Ana Paula Chappuis Chocano ^[2] ; Rodrigo Moreira Bringel-da-Costa ^[3] ; Mariana Domingues Pordeus ^[4] ; Jade Laísa Gordilio Zago ^[5] ; Vinícius Carvalho Porto ^[6]

Afiliação do(s) autor(es)

^[1] University of São Paulo. Bauru School of Dentistry. Department of Prosthodontics - Brasil

^[2] University of São Paulo. Bauru School of Dentistry. Department of Prosthodontics - Brasil

^[3] University of São Paulo. Bauru School of Dentistry. Department of Prosthodontics - Brasil

^[4] University of São Paulo. Bauru School of Dentistry. Department of Prosthodontics - Brasil

^[5] University of São Paulo. Bauru School of Dentistry. Department of Prosthodontics - Brasil

^[6] University of São Paulo. Bauru School of Dentistry. Department of Prosthodontics - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Int. J. Odontostomat.; v. 16, n. 1, p. 68-72, 2022-03-00.

Resumo

ABSTRACT: The aim of this study is to evaluate the effect of two ethyl cyanoacrylate-based adhesives on the growth of Candida albicans biofilms on a heat-polymerized resin, after 7, 14, and 30 days of exposure. Ninety circular (10 x 2 mm) heat-polymerized resin specimens were equally divided into three groups: control, conventional ethyl cyanoacrylate (ECAc), and ethyl cyanoacrylate gel (ECAg). Two layers of 50 µL of each material were applied to the respective groups. C. albicans SC5314 strain was activated and standardized to 107 cells/mL-1. Specimens were immersed in 1 mL of artificial saliva and deposited in 1 mL fungal suspension, washed, and immersed in 1 mL of RPMI for 7, 14, and 30 days. The medium was changed at 48-hour intervals. The final suspension was diluted (10 -1 to 10-4) and deposited on Sabouraud dextrose agar for 48 h at 37 °C. After this period, the colonies were quantified using the CFU/mL calculation. Data were evaluated using one- way ANOVA and Tukey’s test for post-hoc analysis (P=0.05). It was observed that both adhesives significantly reduced (P<0.05) biofilm formation compared to the control at all evaluated periods. In conclusion, an immediate and long-term inhibitory effect on C. albicans biofilm formation was observed. (AU)

• Processo FAPESP: 09/14152-1 - Mecanismos de defesa dos mastócitos contra Candida albicans: participação do receptor Toll-like 2 na produção das citocinas TNF-alfa e IL-10, e nas atividades fagocitária e microbicida
• Beneficiário: Vanessa Soares Lara
• Modalidade de apoio: Auxílio à Pesquisa - Regular