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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Longitudinal Evaluation of Ethyl Cyanoacrylate Adhesives on Candida albicans Biofilm

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Author(s):
Helena Sandrini Venante [1] ; Ana Paula Chappuis Chocano [2] ; Rodrigo Moreira Bringel-da-Costa [3] ; Mariana Domingues Pordeus [4] ; Jade Laísa Gordilio Zago [5] ; Vinícius Carvalho Porto [6]
Total Authors: 6
Affiliation:
[1] University of São Paulo. Bauru School of Dentistry. Department of Prosthodontics - Brasil
[2] University of São Paulo. Bauru School of Dentistry. Department of Prosthodontics - Brasil
[3] University of São Paulo. Bauru School of Dentistry. Department of Prosthodontics - Brasil
[4] University of São Paulo. Bauru School of Dentistry. Department of Prosthodontics - Brasil
[5] University of São Paulo. Bauru School of Dentistry. Department of Prosthodontics - Brasil
[6] University of São Paulo. Bauru School of Dentistry. Department of Prosthodontics - Brasil
Total Affiliations: 6
Document type: Journal article
Source: Int. J. Odontostomat.; v. 16, n. 1, p. 68-72, 2022-03-00.
Abstract

ABSTRACT: The aim of this study is to evaluate the effect of two ethyl cyanoacrylate-based adhesives on the growth of Candida albicans biofilms on a heat-polymerized resin, after 7, 14, and 30 days of exposure. Ninety circular (10 x 2 mm) heat-polymerized resin specimens were equally divided into three groups: control, conventional ethyl cyanoacrylate (ECAc), and ethyl cyanoacrylate gel (ECAg). Two layers of 50 µL of each material were applied to the respective groups. C. albicans SC5314 strain was activated and standardized to 107 cells/mL-1. Specimens were immersed in 1 mL of artificial saliva and deposited in 1 mL fungal suspension, washed, and immersed in 1 mL of RPMI for 7, 14, and 30 days. The medium was changed at 48-hour intervals. The final suspension was diluted (10 -1 to 10-4) and deposited on Sabouraud dextrose agar for 48 h at 37 °C. After this period, the colonies were quantified using the CFU/mL calculation. Data were evaluated using one- way ANOVA and Tukey’s test for post-hoc analysis (P=0.05). It was observed that both adhesives significantly reduced (P<0.05) biofilm formation compared to the control at all evaluated periods. In conclusion, an immediate and long-term inhibitory effect on C. albicans biofilm formation was observed. (AU)

FAPESP's process: 09/14152-1 - Defense mechanisms of mast cells against Candida albicans: involvement of Toll-like 2 receptor in the production of cytokines TNF-alpha and IL-10, and the phagocytic and antimicrobial activities
Grantee:Vanessa Soares Lara
Support Opportunities: Regular Research Grants