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Evaluation of antimicrobial activity of metabolites produced by Streptococcus mutans on Candida albicans: in vitro study

Grant number: 16/05226-5
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Start date: September 01, 2016
End date: August 31, 2018
Field of knowledge:Biological Sciences - Microbiology
Principal Investigator:Juliana Campos Junqueira
Grantee:Mariana de Sá Alves
Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

Streptococcus mutans, often recognized as the main cause of tooth decay in the world, can be found along with Candida albicans in the biofilm. Products of secondary metabolism of the human microbiome are being increasingly studied, because of their direct impact on the host and other microorganisms as well as for their immunomodulatory functions. The aim of this study will be to extract the compounds present in the supernatant of S. mutans culture and see its effects on C. albicans, in vitro. The supernatant of S. mutans culture will be submitted to extraction with ethyl acetate and then the organic solvent will be removed in vacuum, under controlled pressure and temperature. Then, the extract will be dried and weighed, yielding the crude extract. The antimicrobial activity of the crude extract will be tested on C. albicans for in vitro biofilm formation and filamentation. Biofilms will be formed in bottom of 96-well plates for 48 hours and quantified by counting of colony forming units (CFU/mL). The C. albicans filamentation assays will be performed in 24-well plates and analyzed by optical microscopy. The results will be submitted to an exploratory analysis for selection of the most appropriate statistical test for each experiment of this study. (AU)

News published in Agência FAPESP Newsletter about the scholarship:
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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DOS SANTOS, JESSICA DIANE; DE OLIVEIRA FUGISAKI, LUCIANA RUANO; MEDINA, REBECA PREVIATE; SCORZONI, LILIANA; ALVES, MARIANA DE SA; DE BARROS, PATRICIA PIMENTEL; RIBEIRO, FELIPE CAMARGO; FUCHS, BETH BURGWYN; MYLONAKIS, ELEFTHERIOS; SIQUEIRA SILVA, DULCE HELENA; et al. Streptococcus mutansSecreted Products InhibitCandida albicansInduced Oral Candidiasis. FRONTIERS IN MICROBIOLOGY, v. 11, . (16/03395-4, 16/05226-5)
ALVES, MARIANA DE SA; DE OLIVEIRA FUGISAKI, LUCIANA RUANO; DOS SANTOS, JESSICA DIANE; SCORZONI, LILIANA; MEDINA, REBECA PREVIATE; SIQUEIRA SILVA, DULCE HELENA; JUNQUEIRA, JULIANA CAMPOS. Antifungal effects of Streptococcus mutans extract on Candida strains susceptible and resistant to fluconazole: An in vivo study. Medical Mycology, v. 59, n. 7, p. 744-747, . (16/03395-4, 16/05226-5)

Filed patent(s) as a result of this research project

MÉTODO DE OBTENÇÃO DO EXTRATO E FRAÇÕES DO SOBRENADANTE DE S. MUTANS E USO DO EXTRATO E FRAÇÕES DO SOBRENADANTE DE S. MUTANS COMO ANTIFÚNGICO BR102019019528-2 - Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp) . JULIANA CAMPOS JUNQUEIRA / DULCE HELENA SIQUEIRA SILVA / JÉSSICA DIANE DOS SANTOS / LUCIANA RUANO DE OLIVEIRA FUGISAKI / REBECA PREVIATE MEDINA - September 2019, 19