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Antimicrobial activity of metabolites produced by Streptococcus mutans on Candida albicans in in vivo study models

Grant number: 16/03395-4
Support type:Scholarships in Brazil - Master
Effective date (Start): September 01, 2016
Effective date (End): January 31, 2018
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Juliana Campos Junqueira
Grantee:Jéssica Diane dos Santos
Home Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

"In vitro" studies have shown that Streptococcus mutans can produce metabolites capable of inhibiting Candida albicans, becomming interesting the identification and development of new substances for the treatment of oral candidiasis. The aim of this study is to purify and identify the metabolites produced by S. mutans and evaluate their effects on the pathogenicity of C. albicans and on the immune response in "in vivo" models study. The supernatant metabolites of S. mutans culture will be extracted with ethyl acetate and then fractionated and identified by High-performance Liquid Chromatography - Diode Array Detection (HPLC-DAD). Hereafter, the effects of the metabolites will be assessed on experimental candidiasis in Galleria mellonella and in immunosuppressed mice. In experimental infection model with G. mellonella, the effects of the metabolites will be analyzed by survival curve test, quantitation of CFU/ml of C. albicans in hemolymph, phagocytosis and determining of hemocyte density of G. mellonella larvae. The S. mutans metabolite with greater antimicrobial activity against C. albicans in G. mellonella will be selected for the study of oral candidiasis in mice. In this experimental model, the development of candidosis will be evaluated by recovery tests and determination of CFU/ml of C. albicans from the mice's oral cavity, macroscopic and microscopic examination of the tongue dorsum and quantification of cytokines from the serum of the animals. The results of the survival experiments in G. mellonella will be analyzed by log-rank test (Mantel-Cox). The data obtained in the other tests will be submitted to an exploratory analysis for the selection of the most appropriate statistical test for each experiment. In all tests will be used GraphPad Prism 5.0 program, considering a significance level of 5%.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DOS SANTOS, JESSICA DIANE; DE OLIVEIRA FUGISAKI, LUCIANA RUANO; MEDINA, REBECA PREVIATE; SCORZONI, LILIANA; ALVES, MARIANA DE SA; DE BARROS, PATRICIA PIMENTEL; RIBEIRO, FELIPE CAMARGO; FUCHS, BETH BURGWYN; MYLONAKIS, ELEFTHERIOS; SIQUEIRA SILVA, DULCE HELENA; JUNQUEIRA, JULIANA CAMPOS. Streptococcus mutansSecreted Products InhibitCandida albicansInduced Oral Candidiasis. FRONTIERS IN MICROBIOLOGY, v. 11, JUL 15 2020. Web of Science Citations: 0.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SANTOS, Jéssica Diane dos. Atividade antifúngica do extrato bruto e frações de Streptococcus mutans sobre Candida albicans em modelos de estudo in vivo. 2018. 97 f. Master's Dissertation - Universidade Estadual Paulista "Julio de Mesquita Filho" Instituto de Ciência e Tecnologia (Campus de São José dos Campos)..

Please report errors in scientific publications list by writing to: cdi@fapesp.br.