Interaction between Streptococcus mutans and Candida albicans: formation of biofilm in vitro and experimental study on models of invertebrates

• Grant number: 12/15262-8
• Support type: Scholarships in Brazil - Master
• Effective date (Start): November 01, 2012
• Effective date (End): January 31, 2014
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Juliana Campos Junqueira
• Grantee: Júnia Oliveira Barbosa
• Home Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

The interactions between fungi and bacteria are present in nature and has great environmental and medical importance. The proper development in vitro and in vivo models to characterize these interactions is essential for the understanding of the development of diseases and the research of new therapeutic strategies. The aim of this study is to evaluate the microbial interactions between Streptococcus mutans and Candida albicans biofilms models in vitro and in experimental models of invertebrates. At the in vitro study, will be evaluated the influence os S. mutans over the biofilm formation and filamentation capability of the C. albicans. In both tests, will be assessed the direct effects of the cells of S. mutans on C. albicans and also the indirect effects of S. mutans on C. albicans, using only the supernatant of their culture. In addition, tested the effects of S. mutans on C. albicans in different growth phases of bacterial culture (4, 6, 18 e 24h). For the study in vivo, the strains of S. mutans will be inoculated along with C. albicans in larvae of Galleria mellonella for induction of experimental infection. The effects of S. mutans on candidosis experimental analysis will be evaluated by curve by G. mellonella survival, for studies of tissue culture for UFC/mL quantification of C. albicans and histological evaluation of filamentation zone of C. albicans in the tissues of the host. The results of the tests in vitro and in UFC/mL count G. mellonella will be submitted to ANOVA and Tukey's test. The data obtained in the survival curve by G. mellonella will be analyzed by method of Log-rank. In all tests, will be considered a significance level of 5%. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BARBOSA, JUNIA OLIVEIRA; ROSSONI, RODNEI DENNIS; GODINHO VILELA, SIMONE FURGERI; DE ALVARENGA, JANANA ARAUJO; VELLOSO, MARISOL DOS SANTOS; DE AZEVEDO PRATA, MARCIA CRISTINA; CARDOSO JORGE, ANTONIO OLAVO; JUNQUEIRA, JULIANA CAMPOS. Streptococcus mutans Can Modulate Biofilm Formation and Attenuate the Virulence of Candida albicans. PLoS One, v. 11, n. 3 MAR 2 2016. Web of Science Citations: 19.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BARBOSA, Júnia Oliveira. Avaliação dos efeitos de Streptococcus mutans sobre formação de biofilme e morfogênese de Candida albicans in vitro e estudo experimental em Galleria mellonella. 2014. 85 f. Master's Dissertation - Universidade Estadual Paulista "Julio de Mesquita Filho". Instituto de Ciência e Tecnologia.