The Inhibition of Inflammasome by Brazilian Propolis (EPP-AF)

Autor(es)

Hori, Juliana I. ; Zamboni, Dario S. ; Carrao, Daniel B. ; Goldman, Gustavo Henrique ; Berretta, Andresa A.

• Tipo de documento: Artigo Científico
• Fonte: Evidence-based Complementary and Alternative Medicine; v. 2013, p. 11-pg., 2013-01-01.

Resumo

Propolis extracts have gained the attention of consumers and researchers due to their unique chemical compositions and functional properties such as its anti-inflammatory activity. Recently, it was described a complex that is also important in inflammatory processes, named inflammasome. The inflammasomes are a large molecular platform formed in the cell cytosol in response to stress signals, toxins, and microbial infections. Once activated, the inflammasome induces caspase-1, which in turn induces the processing of inflammatory cytokines such as IL-1 beta. and IL-18. So, to understand inflammasomes regulation becomes crucial to treat several disorders including autoinflammatory diseases. Since green propolis extracts are able to regulate inflammatory pathways, this work purpose was to investigate if this extract could also act on inflammasomes regulation. First, the extract was characterized and it demonstrated the presence of important compounds, especially Artepillin C. This extract was effective in reducing the IL-1 beta. secretion in mouse macrophages and this reduction was correlated with a decrease in activation of the protease caspase-1. Furthermore, we found that the extract at a concentration of 30 mu g/mL was not toxic to the cells even after a 18-hour treatment. Altogether, these data indicate that Brazilian green propolis (EPP-AF) extract has a role in regulating the inflammasomes. (AU)

• Processo FAPESP: 06/52867-4 - Reconhecimento de patógenos bacterianos por receptores intracelulares e sua importância no controle da infecção microbiana
• Beneficiário: Dario Simões Zamboni
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 11/50089-2 - Obtenção de um insumo farmacêutico de origem natural para tratamento de candidemias e candidíases
• Beneficiário: Andresa Aparecida Berretta e Silva
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisa Inovativa em Pequenas Empresas - PIPE
• Processo FAPESP: 11/51023-5 - O papel de transportadores de potássio bacterianos no processo de ativação do inflamassoma e na resposta de macrófagos a infecção por Legionella pneumophila
• Beneficiário: Juliana Issa Hori
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado