An evaluation, using the comet assay and the micronucleus test, of the antigenotoxic effects of chlorophyll b in mice

Autor(es)

Serpeloni, Juliana Mara ^[1] ; Grotto, Denise ^[1] ; Aissa, Alexandre Ferro ^[1] ; Mercadante, Adriana Zerlotti ^[2] ; Pires Bianchi, Maria de Lourdes ^[1] ; Greggi Antunes, Lusania Maria ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Anal Clin Toxicol & Bromatol, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto - Brazil

^[2] Univ Estadual Campinas, Fac Engn Alimentos, Dept Ciencia Alimentos, BR-13083862 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS; v. 725, n. 1-2, p. 50-56, OCT 9 2011.
• Citações Web of Science: 12

Resumo

We investigated the effects of the dietary pigment chlorophyll b (CLb) on cisplatin (cDDP)-induced oxidative stress and DNA damage, using the comet assay in mouse peripheral blood cells and the micronucleus (MN) test in bone marrow and peripheral blood cells. We also tested for thiobarbituric acid reactive substances (TBARS) and reduced glutathione (GSH) in liver and kidney tissues, as well as catalase (CAT) activity and GSH in total blood. CLb (0.2 and 0.5 mg/kg b.w.) was administrated by gavage every day for 13 days. On the 14th day of the experiment, 6 mg/kg cDDP or saline was delivered intraperitoneally. Treatment with cDDP led to a significant decrease in DNA migration and an increase in MN frequency in both cell types, bone marrow and peripheral blood cells. In the kidneys of mice treated with cDDP, TBARS levels were increased, whereas GSH levels were depleted in kidney and liver. In mice that were pretreated with CLb and then treated with cDDP, TBARS levels maintained normal concentrations and GSH did not differ from cDDP group. The improvement of oxidative stress biomarkers after CLb pre-treatment was associated with a decrease in DNA damage, mainly for the highest dose evaluated. Furthermore, CLb also slightly reduced the frequency of chromosomal breakage and micronucleus formation in mouse bone marrow and peripheral blood cells. These results show that pre-treatment with CLb attenuates cDDP-induced oxidative stress, chromosome instability, and lipid peroxidation. (C) 2011 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 10/05096-8 - Atividade antigenotóxica de compostos da dieta e sua influência na expressão de genes de resposta ao estresse oxidativo
• Beneficiário: Lusânia Maria Greggi Antunes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 05/59552-6 - Avaliação integrada da estabilidade e propriedades funcionais de pigmentos naturais de alimentos
• Beneficiário: Adriana Zerlotti Mercadante
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 08/06793-4 - Atividade antigenotóxica de compostos da dieta e sua influência na expressão de genes de resposta ao estresse oxidativo
• Beneficiário: Juliana Mara Serpeloni
• Modalidade de apoio: Bolsas no Brasil - Doutorado