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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

An evaluation, using the comet assay and the micronucleus test, of the antigenotoxic effects of chlorophyll b in mice

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Author(s):
Serpeloni, Juliana Mara [1] ; Grotto, Denise [1] ; Aissa, Alexandre Ferro [1] ; Mercadante, Adriana Zerlotti [2] ; Pires Bianchi, Maria de Lourdes [1] ; Greggi Antunes, Lusania Maria [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Dept Anal Clin Toxicol & Bromatol, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto - Brazil
[2] Univ Estadual Campinas, Fac Engn Alimentos, Dept Ciencia Alimentos, BR-13083862 Campinas, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS; v. 725, n. 1-2, p. 50-56, OCT 9 2011.
Web of Science Citations: 12
Abstract

We investigated the effects of the dietary pigment chlorophyll b (CLb) on cisplatin (cDDP)-induced oxidative stress and DNA damage, using the comet assay in mouse peripheral blood cells and the micronucleus (MN) test in bone marrow and peripheral blood cells. We also tested for thiobarbituric acid reactive substances (TBARS) and reduced glutathione (GSH) in liver and kidney tissues, as well as catalase (CAT) activity and GSH in total blood. CLb (0.2 and 0.5 mg/kg b.w.) was administrated by gavage every day for 13 days. On the 14th day of the experiment, 6 mg/kg cDDP or saline was delivered intraperitoneally. Treatment with cDDP led to a significant decrease in DNA migration and an increase in MN frequency in both cell types, bone marrow and peripheral blood cells. In the kidneys of mice treated with cDDP, TBARS levels were increased, whereas GSH levels were depleted in kidney and liver. In mice that were pretreated with CLb and then treated with cDDP, TBARS levels maintained normal concentrations and GSH did not differ from cDDP group. The improvement of oxidative stress biomarkers after CLb pre-treatment was associated with a decrease in DNA damage, mainly for the highest dose evaluated. Furthermore, CLb also slightly reduced the frequency of chromosomal breakage and micronucleus formation in mouse bone marrow and peripheral blood cells. These results show that pre-treatment with CLb attenuates cDDP-induced oxidative stress, chromosome instability, and lipid peroxidation. (C) 2011 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 05/59552-6 - Integrated evaluation of the stability and functional properties of natural pigments of foods
Grantee:Adriana Zerlotti Mercadante
Support type: Research Projects - Thematic Grants
FAPESP's process: 10/05096-8 - Antigenotoxic activity of compounds in diet and its effects in the expression of genes in response to oxidative stress
Grantee:Lusânia Maria Greggi Antunes
Support type: Regular Research Grants
FAPESP's process: 08/06793-4 - Antigenotoxic activity of compounds in the diet and its effect in the expression of genes in response to oxidative stress
Grantee:Juliana Mara Serpeloni
Support type: Scholarships in Brazil - Doctorate