| Texto completo | |
| Autor(es): |
Nascimento, Roberto de Paula do
;
Rizzato, Julia Soto
;
Polezi, Gabriele
;
Boughanem, Hatim
;
Williams, Non Gwenllian
;
Borguini, Renata Galhardo
;
Santiago, Manuela Cristina Pessanha de Araujo
;
Marostica Junior, Mario Roberto
;
Parry, Lee
Número total de Autores: 9
|
| Tipo de documento: | Artigo Científico |
| Fonte: | PLANTS-BASEL; v. 13, n. 20, p. 18-pg., 2024-10-01. |
| Resumo | |
Jaboticaba (Myrciaria jaboticaba) is a recognizable and unique crop from Brazil. The fruit's byproducts are currently being studied, given their bioactive composition and promising anti-cancer potential. It is not evident, however, if different harvesting seasons can modify the chemical profile and antioxidant capacity of jaboticaba fruit fractions. Furthermore, as there is limited data for jaboticaba's anti-proliferative effects, additional assessments are required to improve the robustness of these findings. Therefore, this study aimed to determine the composition of the peel of jaboticaba collected in two periods (May-off-season, sample 1-and August-October-peak season, sample 2) and test the peel's richest anthocyanin sample against colorectal cancer (CRC) cell lines. To accomplish this, proximate, spectrophotometric, and chromatographic analyses were performed in two freeze-dried samples; and anti-proliferative and/or colony-forming assays were carried out in Caco-2, HT29, and HT29-MTX cells. As a result, sample 2 showed the highest levels of polyphenols overall, including flavonoids and anthocyanins. This sample displayed significative higher contents of cyanidin-3-O-glucoside (48%) and delphinidin-3-O-glucoside (105%), in addition to a superior antioxidant capacity (23% higher). Sample 1 showed higher amounts of total protein, gallic acid (20% higher), and specific carotenoids. An aqueous extract from sample 2 was tested against CRC, showing anti-proliferative effects for Caco-2 cells at 1 and 2 mg/mL concentrations, with IC50 values of 1.2-1.3 mg/mL. Additionally, the extract was able to inhibit cell colony formation when tested at both low and high concentrations. In conclusion, jaboticaba collected in the main season stands out regarding its polyphenol composition and holds potential against cancer cell growth. (AU) | |
| Processo FAPESP: | 22/10485-0 - Pó liofilizado da casca da jabuticaba na prevenção do câncer colorretal: mecanismos em camundongos 'wild type' e geneticamente modificados |
| Beneficiário: | Roberto de Paula do Nascimento |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Doutorado |
| Processo FAPESP: | 22/09493-9 - Estudos in vitro do metaboloma de antocianinas de frutas visando entender seus mecanismos de ação contra a Obesidade |
| Beneficiário: | Juliano Lemos Bicas |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 19/03228-9 - Efeitos do consumo preventivo do pó liofilizado da casca da jabuticaba em camundongos BALB/c com Câncer Colorretal induzido por azoximetano/dextran sulfato de sódio |
| Beneficiário: | Roberto de Paula do Nascimento |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 20/00414-3 - Efeitos preventivos do pó liofilizado da casca da jabuticaba em modelo experimental de câncer colorretal: avaliação clínica e histofisiopatológica |
| Beneficiário: | Julia Soto Rizzato |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |