Identification of candidate lethal haplotypes and genomic association with post-natal mortality and reproductive traits in Nellore cattle

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Schmidt, Patricia Iana ; Mota, Lucio Flavio Macedo ; Fonseca, Larissa Fernanda Simielli ; dos Santos Silva, Danielly Beraldo ; Frezarim, Gabriela Bonfa ; Arikawa, Leonardo Machestropa ; de Abreu Santos, Daniel Jordan ; Magalhes, Ana Fabricia Braga ; Cole, John Bruce ; Carvalheiro, Roberto ; de Oliveira, Henrique Nunes ; Null, Daniel Jacob ; VanRaden, Paul ; Ma, Li ; de Albuquerque, Lucia Galvo

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 13, n. 1, p. 12-pg., 2023-06-27.

Resumo

The wide use of genomic information has enabled the identification of lethal recessive alleles that are the major genetic causes of reduced conception rates, longer calving intervals, or lower survival for live-born animals. This study was carried out to screen the Nellore cattle genome for lethal recessive haplotypes based on deviation from the expected population homozygosity, and to test SNP markers surrounding the lethal haplotypes region for association with heifer rebreeding (HR), post-natal mortality (PNM) and stayability (STAY). This approach requires genotypes only from apparently normal individuals and not from affected embryos. A total of 62,022 animals were genotyped and imputed to a high-density panel (777,962 SNP markers). Expected numbers of homozygous individuals were calculated, and the probabilities of observing 0 homozygotes was obtained. Deregressed genomic breeding values [(G)EBVs] were used in a GWAS to identify candidate genes and biological mechanisms affecting HR, STAY and PNM. In the functional analyses, genes within 100 kb down and upstream of each significant SNP marker, were researched. Thirty haplotypes had high expected frequency, while no homozygotes were observed. Most of the alleles present in these haplotypes had a negative mean effect for PNM, HR and STAY. The GWAS revealed significant SNP markers involved in different physiological mechanisms, leading to harmful effect on the three traits. The functional analysis revealed 26 genes enriched for 19 GO terms. Most of the GO terms found for biological processes, molecular functions and pathways were related to tissue development and the immune system. More phenotypes underlying these putative regions in this population could be the subject of future investigation. Tests to find putative lethal haplotype carriers could help breeders to eliminate them from the population or manage matings in order to avoid homozygous. (AU)

• Processo FAPESP: 09/16118-5 - Ferramentas genômicas no melhoramento genético de características de importância econômica direta em bovinos da raça Nelore
• Beneficiário: Lucia Galvão de Albuquerque
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 17/10630-2 - Aspectos genéticos da qualidade, eficiência e sustentabilidade da produção de carne em animais da raça Nelore
• Beneficiário: Lucia Galvão de Albuquerque
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/20026-8 - EMU concedido no processo 2017/10630-2: servidor
• Beneficiário: Lucia Galvão de Albuquerque
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 18/17812-1 - Identificação de candidatos a haplótipos letais em bovinos Nelore
• Beneficiário: Patrícia Iana Schmidt
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 19/10123-9 - Identificação de eventos de recombinação em bovinos Nelore
• Beneficiário: Patrícia Iana Schmidt
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Mestrado
• Processo FAPESP: 21/09942-5 - Estimativa da variância da diversidade gamética para características economicamente importantes de bovinos Nelore
• Beneficiário: Patrícia Iana Schmidt
• Modalidade de apoio: Bolsas no Brasil - Doutorado