Foliar Application of SeNPs for Rice Biofortification: a Comparative Study with Selenite and Speciation Assessment

Autor(es)

Freire, Bruna Moreira ; Lange, Camila Neves ; Augusto, Caroline Cristine ; Onwuatu, Fernanda Ribeiro ; Rodrigues, Gustavo Dell'Arno Principe ; Pieretti, Joana Claudio ; Seabra, Amedea Barozzi ; Batista, Bruno Lemos

• Tipo de documento: Artigo Científico
• Fonte: ACS AGRICULTURAL SCIENCE & TECHNOLOGY; v. 5, n. 1, p. 14-pg., 2024-12-17.

Resumo

A significant portion of the global population lacks access to a balanced diet, leading to widespread micronutrient deficiencies. Selenium (Se) deficiency affects approximately 1 billion people worldwide, and agronomic biofortification of food crops using inorganic Se fertilizers or Se nanoparticles (SeNPs) has emerged as a potential solution. However, to ensure food safety, it is critical to assess whether nonbioavailable or toxic Se species are formed when SeNPs are introduced into plants. In this study, pot experiments with rice plants (Oryza sativa L.) were conducted to evaluate the effects of foliar applications of selenite (Se(IV)) and SeNPs on Se uptake, translocation, and speciation. Plant growth, chemical, and biochemical parameters were evaluated. Selenium accumulation and speciation were determined using inductively coupled plasma mass spectrometry (ICP-MS) and high-performance liquid chromatography coupled with ICP-MS (HPLC-ICP-MS). The results demonstrated that SeNP treatment did not adversely affect plant growth, grain yield, and oxidative stress or significantly increase the inorganic Se content in rice grains. From a nutritional perspective, grains biofortified with SeNPs had the potential to meet 100% of the recommended daily Se intake. Meanwhile, Se(IV) was more efficient for grain biofortification but increased the concentration of inorganic Se in rice grains by 141% compared to the control group. Regardless of the Se species applied, rice fertilization increased the proportion of selenomethionine while it reduced selenocysteine in grains. The treatment with SeNPs did not compromise the nutritional quality of rice grains but increased As content from 175 to 210 mu g kg-1, which remains below the maximum allowable limit of 350 mu g kg-1 for husked rice. The foliar application of SeNPs enables the production of Se-enriched rice with Se levels controlled within a safe range for human consumption and without significantly altering inorganic Se concentrations. This approach offers a viable strategy for addressing Se deficiency through biofortified rice. (AU)

• Processo FAPESP: 16/10060-9 - Equipamento multi-usuário (EMU) concedido no Processo FAPESP 2014/05151-0: espectrômetro de massas com plasma indutivamente acoplado hifenado a um cromatógrafo a líquido de alta eficiência (HPLC-ICP-MS)
• Beneficiário: Bruno Lemos Batista
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 22/14645-2 - Hidrogéis e próteses vasculares liberadores de óxido nítrico para aplicações cardiovasculares
• Beneficiário: Marcelo Ganzarolli de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 20/00284-2 - Avaliação do uso de nanopartículas de selênio para biofortificação de grãos de arroz
• Beneficiário: Bruna Moreira Freire
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 22/04254-6 - Avaliação do uso de spICP-MS para dimensionamento de SeNPs internalizadas por plantas de arroz e localização do Se acumulado por LA-ICP-MS
• Beneficiário: Bruna Moreira Freire
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 14/05151-0 - Arsênio e arroz: monitoramento e estudos de (bio)remediação para segurança alimentar
• Beneficiário: Bruno Lemos Batista
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores