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Pectin Methylesterase Inactivation Kinetics in the Microwave Pasteurization of Mango Pulp and Nectar

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Autor(es):
Cavalcante, Tiago Augusto Bulhoes Bezerra ; Tadini, Carmen Cecilia ; Gut, Jorge Andrey Wilhelms
Número total de Autores: 3
Tipo de documento: Artigo Científico
Fonte: Brazilian Archives of Biology and Technology; v. 68, p. 13-pg., 2025-01-01.
Resumo

This study investigates the thermal inactivation kinetics of pectin methylesterase (PME) in mango pulp and nectar (Palmer variety), using conventional and microwave heating. The aim was to provide practical processing parameters and examine potential non-thermal effects associated with microwave treatment. To ensure a proper comparison between heating technologies, similar temperature histories were obtained in paired experiments (conventional hot water bath vs. microwave reactor), with homogeneous sample temperature and full temperature history recorded (heating, holding, cooling). Samples (10 mL) were processed with temperatures between 50 to 80 degrees C and holding times between 2 and 101 s. A two-component first-order kinetic model with five parameters was adjusted (simultaneously solving the numerical integration of the temperature histories). For a reference temperature of 65 degrees C the D-values obtained were 4.964<middle dot>10-2 s and 92.57 s (conventional) and 2.691<middle dot>10-3 sand 79.82 s (microwave), with 55% of the initial acidity due to the thermolabile fraction. Predicted inactivation curves show that this thermolabile fraction is rapidly inactivated at 65 degrees C, while 1-2 min are required to bring the residual activity from the thermostable fraction down to 5%. PME showed to be more resistant in the mango pulp, requiring nearly 2 min to reach 10% at 75 degrees C. The comparison between conventional and microwave heating indicated the presence of non-thermal microwave effects at 55 and 60 degrees C; however, thermal effects dominate at practical processing temperatures, with no significant difference. (AU)

Processo FAPESP: 12/04073-0 - Determinação experimental de propriedades dielétricas de alimentos líquidos e pastosos visando o tratamento térmico contínuo por tecnologia de micro-ondas
Beneficiário:Carmen Cecilia Tadini
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 13/07914-8 - FoRC - Centro de Pesquisa em Alimentos
Beneficiário:Bernadette Dora Gombossy de Melo Franco
Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
Processo FAPESP: 14/17534-0 - Avaliação dos efeitos do processamento por micro-ondas focalizadas sobre alimentos líquidos
Beneficiário:Jorge Andrey Wilhelms Gut
Modalidade de apoio: Auxílio à Pesquisa - Regular