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Dos-Santos, Joao Conrado Khouri
;
Silva-Filho, Joao Luiz
;
Judice, Carla C.
;
Kayano, Ana Carolina Andrade Vitor
;
Aliberti, Julio
;
Khouri, Ricardo
;
de Lima, Diogenes S.
;
Nakaya, Helder
;
Lacerda, Marcus Vinicius Guimaraes
;
De Paula, Erich Vinicius
;
Lopes, Stefanie Costa Pinto
;
Costa, Fabio Trindade Maranhao
Número total de Autores: 12
|
| Tipo de documento: | Artigo Científico |
| Fonte: | PLoS Neglected Tropical Diseases; v. 14, n. 7, p. 14-pg., 2020-07-01. |
| Resumo | |
Author summary Endothelial cell activation is a key process in the pathogenesis ofPlasmodium vivaxmalaria. Platelets are classically involved in endothelial cell activation in several diseases, but their role in the context of vivax malaria remains unclear. Thrombocytopenia is the most common hematological disturbance inP.vivax-infected patients, and platelets have been implicated in parasitemia control. In this work, we studied the activation of platelets in association with endothelial cell activation in vivax malaria. Platelets retrieved from infected peripheral blood were non-activated when analyzed by flow cytometry; however, they displayed higher mean volume and significantly reduced counts. We also found higher levels of circulating factors associated with platelet activation (especially soluble CD40L), thrombopoiesis and endothelial cell activation in infected patients. Further, through pair-wise correlation and clustering analysis, we found a subgroup of patients showing significant associations between markers of platelet and endothelial activation in a pattern different from that of endemic controls. Collectively, our findings indicate a role of platelets in endothelial cell activation in vivax malaria and indicate a heterogeneous host response in the setting of uncomplicated disease, a finding to be further explored in future studies. Platelets drive endothelial cell activation in many diseases. However, if this occurs inPlasmodium vivaxmalaria is unclear. As platelets have been reported to be activated and to play a role in inflammatory response during malaria, we hypothesized that this would correlate with endothelial alterations during acute illness. We performed platelet flow cytometry of PAC-1 and P-selectin. We measured platelet markers (CXCL4, CD40L, P-selectin, Thrombopoietin, IL-11) and endothelial activation markers (ICAM-1, von Willebrand Factor and E-selectin) in plasma with a multiplex-based assay. The values of each mediator were used to generate heatmaps, K-means clustering and Principal Component analysis. In addition, we determined pair-wise Pearson's correlation coefficients to generate correlation networks. Platelet counts were reduced, and mean platelet volume increased in malaria patients. The activation of circulating platelets in flow cytometry did not differ between patients and controls. CD40L levels (Median [IQ]: 517 [406-651] vs. 1029 [732-1267] pg/mL,P= 0.0001) were significantly higher in patients, while P-selectin and CXCL4 showed a nonsignificant trend towards higher levels in patients. The network correlation approach demonstrated the correlation between markers of platelet and endothelial activation, and the heatmaps revealed a distinct pattern of activation in two subsets ofP.vivaxpatients when compared to controls. Although absolute platelet activation was not strong in uncomplicated vivax malaria, markers of platelet activity and production were correlated with higher endothelial cell activation, especially in a specific subset of patients. (AU) | |
| Processo FAPESP: | 17/18611-7 - Desenvolvimento de novas ferramentas para busca e validação de alvos moleculares para terapia contra Plasmodium vivax |
| Beneficiário: | Fabio Trindade Maranhão Costa |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 16/12855-9 - Mecanismo de ação da lipoxina na proteção do desenvolvimento da malária cerebral experimental |
| Beneficiário: | João Luiz da Silva Filho |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 12/16525-2 - Plasmodium vivax: patogênese e infectividade |
| Beneficiário: | Fabio Trindade Maranhão Costa |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |