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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Long-term melatonin treatment reduces ovarian mass and enhances tissue antioxidant defenses during ovulation in the rat

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Autor(es):
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Chuffa, L. G. A. [1, 2] ; Amorim, J. P. A. [1, 2] ; Teixeira, G. R. [1] ; Mendes, L. O. [1, 2] ; Fioruci, B. A. [1, 2] ; Pinheiro, P. F. F. [1] ; Seiva, F. R. F. [3] ; Novelli, E. L. B. [3] ; Mello Junior, W. [1] ; Martinez, M. [4] ; Martinez, F. E. [1]
Número total de Autores: 11
Afiliação do(s) autor(es):
[1] Univ Estadual Paulista, Inst Biociencias, Dept Anat, BR-18618000 Botucatu, SP - Brazil
[2] Univ Estadual Campinas, Inst Biol, Programa Posgrad Biol Celular & Estrutural, Campinas, SP - Brazil
[3] Univ Estadual Paulista, Inst Biociencias, Dept Quim & Bioquim, BR-18618000 Botucatu, SP - Brazil
[4] Univ Fed Sao Carlos, Dept Morfol & Patol, BR-13560 Sao Carlos, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: Brazilian Journal of Medical and Biological Research; v. 44, n. 3, p. 217-223, MAR 2011.
Citações Web of Science: 23
Resumo

Melatonin regulates the reproductive cycle, energy metabolism and may also act as a potential antioxidant indoleamine. The present study was undertaken to investigate whether long-term melatonin treatment can induce reproductive alterations and if it can protect ovarian tissue against lipid peroxidation during ovulation. Twenty-four adult female Wistar rats, 60 days old (+/- 250-260 g), were randomly divided into two equal groups. The control group received 0.3 mL 0.9% NaCl + 0.04 mL 95% ethanol as vehicle, and the melatonin-treated group received vehicle + melatonin (100 mu g.100 g body weight(-1).day(-1)) both intraperitoneally daily for 60 days. All animals were killed by decapitation during the morning estrus at 4: 00 am. Body weight gain and body mass index were reduced by melatonin after 10 days of treatment (P < 0.05). Also, a marked loss of appetite was observed with a fall in food intake, energy intake (melatonin 51.41 +/- 1.28 vs control 57.35 +/- 1.34 kcal/day) and glucose levels (melatonin 80.3 +/- 4.49 vs control 103.5 +/- 5.47 mg/dL) towards the end of treatment. Melatonin itself and changes in energy balance promoted reductions in ovarian mass (20.2%) and estrous cycle remained extensive (26.7%), arresting at diestrus. Regarding the oxidative profile, lipid hydroperoxide levels decreased after melatonin treatment (6.9%) and total antioxidant substances were enhanced within the ovaries (23.9%). Additionally, melatonin increased superoxide dismutase (21.3%), catalase (23.6%) and glutathione-reductase (14.8%) activities and the reducing power (10.2% GSH/GSSG ratio). We suggest that melatonin alters ovarian mass and estrous cyclicity and protects the ovaries by increasing superoxide dismutase, catalase and glutathione-reductase activities. (AU)

Processo FAPESP: 07/59967-7 - Ação da melatonina sobre os receptores estrogênicos ER alfa e ER beta e progestagênicos PRA e PRB nos órgãos genitais femininos internos e o estresse oxidativo nos ovários de ratas adultas UChB (consumidoras voluntárias de etanol a 10%)
Beneficiário:Luiz Gustavo de Almeida Chuffa
Modalidade de apoio: Bolsas no Brasil - Doutorado