Central NOS inhibition differentially affects vasopressin gene expression in hypothalamic nuclei in septic rats

Autor(es)

Oliveira-Pelegrin, Gabriela Ravanelli ^[1] ; de Azevedo, Sergio Vicente ^[2] ; Yao, Song Tieng ^[3] ; Murphy, David ^[3] ; Alves Rocha, Maria Jose ^[1]

Afiliação do(s) autor(es)

^[1] USP, Fac Odontol Ribeirao Preto, Dept MEF, BR-14040904 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Ribeirao Preto, SP - Brazil

^[3] Univ Bristol, Henry Wellcome Labs Integrat Neurosci & Endocrino, Bristol, Avon - England

• Tipo de documento: Artigo Científico
• Fonte: Journal of Neuroimmunology; v. 227, n. 1-2, p. 80-86, OCT 8 2010.
• Citações Web of Science: 14

Resumo

Our aim was to investigate the effect of central NOS inhibition on hypothalamic arginine vasopressin (AVP) gene expression, hormone release and on the cardiovascular response during experimental sepsis. Male Wistar rats were intracerebroventricularly injected with the non-selective NO synthase (NOS) inhibitor (L-NAME) or aminoguanidine, a selective inhibitor of the inducible isoform (iNOS). After 30 min. sepsis was induced by cecal ligation and puncture (CLP) causing an increase in heart rate (HR), as well as a reduction in median arterial pressure (MAP) and AVP expression ratio (AVP(R)), mainly in the supraoptic nucleus. AVP plasma levels (AVP(P)) increased in the early but not in the late phase of sepsis. L-NAME pretreatment increased MAP but did not change HR. It also resulted in an increase in AVP(P) at all time points, except 24 h, when it returned to basal levels. AVP(R), however remained reduced in both nuclei. Aminoguanidine pretreatment resulted in increased MAP in the early phase and higher AVP(R) in the supraoptic, but not in the paraventricular nucleus, while AVP(P) remained elevated at all time points. We suggest that increased central NO production, mainly inducible NOS-derived, reduces AVP gene expression differentially in supraoptic and paraventricular nuclei, and that this may contribute to low AVP plasma levels and hypotension in the late phase of sepsis. (c) 2010 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 07/08532-0 - Alterações neuroendócrinas durante sepse experimental induzida por ligação cecal e punção
• Beneficiário: Maria José Alves da Rocha
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 05/50162-0 - Avaliação da participação do óxido nítrico na síntese de vasopressina e ocitocina durante choque séptico experimental induzido por ligadura e perfuração cecal
• Beneficiário: Gabriela Ravanelli de Oliveira Pelegrin
• Modalidade de apoio: Bolsas no Brasil - Doutorado