Role of central NO-cGMP pathway in vasopressin and oxytocin gene expression during sepsis

Autor(es)

Oliveira-Pelegrin, Gabriela Ravanelli ^[1] ; Aguila, Fabio Alves ^[1] ; Basso, Paulo Jose ^[1] ; Alves Rocha, Maria Jose ^[1]

Afiliação do(s) autor(es)

^[1] USP, Fac Odontol Ribeirao Preto, Dept MEF, BR-14040904 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Peptides; v. 31, n. 10, p. 1847-1852, OCT 2010.
• Citações Web of Science: 19

Resumo

Sepsis induces massive production of inflammatory mediators, such as nitric oxide (NO), and causes neuroendocrine and cardiovascular alterations. This study investigates the involvement of the central NO-cGMP pathway in arginine vasopressin (AVP) and oxytocin (OXY) gene expression during sepsis induced by cecal ligation and puncture (CLP). Male Wistar rats received an i.c.v. injection of ODQ (0.25 mu g/mu L), a selective inhibitor of the heme site of soluble guanylate cyclase, or of 1% dymethilsulfoxide (DMSO), as vehicle. Thirty minutes after the injections, sepsis was induced by cecal ligation and puncture or the animals were sham operated. The ODQ pre-treatment did not alter the progressive NO increase observed after CLP. In the supraoptic nucleus (SON), this pretreatment increased the relative gene expression ratio of AVP and OXY in the initial phase of sepsis, but in the late phase, the gene expression of both hormones was reduced. In the paraventricular nucleus (PVN), soluble guanylate cyclase inhibition caused an even larger decrease in the relative gene expression ratio of AVP and OXY during sepsis. These results are indicative of a role of the NO-cGMP pathway in hormonal synthesis in the SON and PVN of the hypothalamus during polymicrobial sepsis. (C) 2010 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 07/08532-0 - Alterações neuroendócrinas durante sepse experimental induzida por ligação cecal e punção
• Beneficiário: Maria José Alves da Rocha
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 05/50162-0 - Avaliação da participação do óxido nítrico na síntese de vasopressina e ocitocina durante choque séptico experimental induzido por ligadura e perfuração cecal
• Beneficiário: Gabriela Ravanelli de Oliveira Pelegrin
• Modalidade de apoio: Bolsas no Brasil - Doutorado