Comparison of the photodynamic fungicidal efficacy of methylene blue, toluidine blue, malachite green and low-power laser irradiation alone against Candida albicans

Autor(es)

Souza, Rodrigo C. ^[1] ; Junqueira, Juliana Campos ^[1] ; Rossoni, Rodnei D. ^[1] ; Pereira, Cristiane A. ^[1] ; Munin, Egberto ^[2] ; Jorge, Antonio O. C. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Sch Dent Sao Jose dos Campos, Dept Biosci & Oral Diag, BR-12245000 Sao Jose Dos Campos, SP - Brazil

^[2] Camilo Castelo Branco Univ UNICASTELO, Sao Jose Dos Campos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Medical Science; v. 25, n. 3, p. 385-389, MAY 2010.
• Citações Web of Science: 84

Resumo

This study was to evaluate specific effects of photodynamic therapy (energy density 15.8 J/cm(2), 26.3 J/cm(2) and 39.5 J/cm(2)) using methylene blue, toluidine blue and malachite green as photosensitizers and low-power laser irradiation on the viability of Candida albicans. Suspensions of C. albicans containing 10(6) cells/ml were standardized in a spectrophotometer. For each dye, 120 assays, divided into four groups according to the following experimental conditions, were carried out: laser irradiation in the presence of the photosensitizer; laser irradiation only; treatment with the photosensitizer only; no exposure to laser light or photosensitizer. Next, serial dilutions were prepared and seeded onto Sabouraud dextrose agar for the determination of the number of colony-forming units per milliliter (CFU/ml). The results were subjected to analysis of variance and the Tukey test (P < 0.05). Photodynamic therapy using the photosensitizers tested was effective in reducing the number of C. albicans.. The number of CFU/ml was reduced by between 0.54 log(10) and 3.07 log(10) and depended on the laser energy density used. Toluidine blue, methylene blue and malachite green were effective photosensitizers in antimicrobial photodynamic therapy against C. albicans, as was low-power laser irradiation alone. (AU)

• Processo FAPESP: 06/54896-1 - Fotossensibilizacao de candida albicans por radiacao laser de baixa intensidade.
• Beneficiário: Rodrigo Cembranelli de Souza
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica