| Texto completo | |
| Autor(es): Mostrar menos - |
Pavan, Marilene
[1, 2]
;
Ruiz, Viviane F.
[1]
;
Silva, Fabio A.
[1, 2]
;
Sobreira, Tiago J.
[1]
;
Cravo, Roberta M.
[2]
;
Vasconcelos, Michelle
[1, 2]
;
Marques, Livia P.
[1]
;
Mesquita, Sonia M. F.
[3]
;
Krieger, Jose E.
[1]
;
Lopes, Antonio A. B.
[3]
;
Oliveira, Paulo S.
[1]
;
Pereira, Alexandre C.
[1]
;
Xavier-Neto, Jose
[1]
Número total de Autores: 13
|
| Afiliação do(s) autor(es): | [1] InCor HC FMUSP, Lab Genet & Cardiol Mol, Sao Paulo - Brazil
[2] ICB USP, Dept Biol Celular & Desenvolvimento, Sao Paulo - Brazil
[3] InCor HC FMUSP, Unidade Clin Cardiol Pediat & Cardiopatias Congen, Sao Paulo - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BMC MEDICAL GENETICS; v. 10, NOV 3 2009. |
| Citações Web of Science: | 21 |
| Resumo | |
Background: Signaling by the vitamin A-derived morphogen retinoic acid (RA) is required at multiple steps of cardiac development. Since conversion of retinaldehyde to RA by retinaldehyde dehydrogenase type II (ALDH1A2, a.k.a RALDH2) is critical for cardiac development, we screened patients with congenital heart disease (CHDs) for genetic variation at the ALDH1A2 locus. Methods: One-hundred and thirty-three CHD patients were screened for genetic variation at the ALDH1A2 locus through bi-directional sequencing. In addition, six SNPs (rs2704188, rs1441815, rs3784259, rs1530293, rs1899430) at the same locus were studied using a TDT-based association approach in 101 CHD trios. Observed mutations were modeled through molecular mechanics (MM) simulations using the AMBER 9 package, Sander and Pmemd programs. Sequence conservation of observed mutations was evaluated through phylogenetic tree construction from ungapped alignments containing ALDH8 s, ALDH1Ls, ALDH1 s and ALDH2 s. Trees were generated by the Neighbor Joining method. Variations potentially affecting splicing mechanisms were cloned and functional assays were designed to test splicing alterations using the pSPL3 splicing assay. Results: We describe in Tetralogy of Fallot (TOF) the mutations Ala151Ser and Ile157Thr that change non-polar to polar residues at exon 4. Exon 4 encodes part of the highly-conserved tetramerization domain, a structural motif required for ALDH oligomerization. Molecular mechanics simulation studies of the two mutations indicate that they hinder tetramerization. We determined that the SNP rs16939660, previously associated with spina bifida and observed in patients with TOF, does not affect splicing. Moreover, association studies performed with classical models and with the transmission disequilibrium test (TDT) design using single marker genotype, or haplotype information do not show differences between cases and controls. Conclusion: In summary, our screen indicates that ALDH1A2 genetic variation is present in TOF patients, suggesting a possible causal role for this gene in rare cases of human CHD, but does not support the hypothesis that variation at the ALDH1A2 locus is a significant modifier of the risk for CHD in humans. (AU) | |
| Processo FAPESP: | 02/11340-2 - Estabelecimento de modelos para a identificação de mecanismos reguladores da expressão do gene RALDH2 |
| Beneficiário: | Roberta Mascioli Cravo |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 01/14238-1 - Sinalização por ácido retinóico e cardiopatias congênitas humanas |
| Beneficiário: | Viviane Felgueiras Ruiz |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |
| Processo FAPESP: | 03/09998-2 - Formação dos compartimentos cardíacos de influxo e efluxo no embrião de vertebrados e a sinalização pelo ácido retinoico: um estudo de mapa do destino |
| Beneficiário: | Michelle Vasconcelos |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 00/03072-2 - Controle molecular da morfogenia das camaras cardiacas. |
| Beneficiário: | Fabio Alex da Silva |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 04/15704-4 - Controle da expressão do gene da RALDH2 durante o desenvolvimento: uma abordagem filogenética |
| Beneficiário: | Roberta Mascioli Cravo |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |
| Processo FAPESP: | 04/11606-8 - Identificação de variantes genéticas em um grupo de genes da via de síntese e sinalização do ácido retinóico - RXRAlpha e CYP26 - em cardiopatias congênitas |
| Beneficiário: | Marilene Elizabete Pavan Rodrigues |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 01/00009-0 - Estudo integrado da hipertensão arterial: caracterização molecular e funcional do sistema cardiovascular |
| Beneficiário: | Eduardo Moacyr Krieger |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |