Evidence for mediation of nociception by injection of the NK-3 receptor agonist, senktide, into the dorsal periaqueductal gray of rats

Autor(es)

Bassi, Gabriel S. ^{[1, 2]} ; Broiz, Ana C. ^{[1, 2]} ; Gomes, Margarete Z. ^{[1, 2]} ; Brandao, Marcus L. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] INeC, BR-14040901 Ribeirao Preto, SP - Brazil

^[2] FFCLRP, Lab Psicobiol, BR-14049901 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Psychopharmacology; v. 204, n. 1, p. 13-24, MAY 2009.
• Citações Web of Science: 8

Resumo

Ultrasound vocalizations (USVs) at approximately 22 kHz are usual components of the defensive response of rats. However, depending on the neural substrate that is activated, such as the dorsal periaqueductal gray (dPAG), USV emissions may be reduced. Activation of neurokinin-1 (NK-1)-mediated mechanisms of the dPAG causes analgesia, reduced 22 kHz USVs, and anxiogenic-like effects in rats exposed to the elevated plus maze (EPM). Involvement of other types of neurokinin receptors in this activation has not yet been evaluated. The present study examined whether local injections of the selective NK-3 agonist senktide (1-100 pmol/0.2 mu L) into the dPAG can (1) cause anxiogenic effects in the EPM, (2) influence novelty-induced 22 kHz USVs, or (3) change nociceptive reactivity in the tail-flick test. Senktide elicited a significant increase in exploratory behavior, an effect accompanied by hyperalgesia and an increase in the number of 22 kHz USVs. The nociceptive effects, increased locomotor activity, and USV emissions elicited by local injections of senktide (50 pmol/0.2 mu L) were reduced by prior injections of the selective NK-3 receptor antagonist SB222200 (50 pmol/0.2 mu L) into the dPAG. These findings show that NK-3 receptors in the dPAG mediate nociceptive responses in this area, contrasting with the known fear-related processes mediated by NK-1 receptors in the dPAG. Both hyperalgesia and fear-related processes are accompanied by emissions of 22 kHz USVs. (AU)

• Processo FAPESP: 06/06354-5 - Psicobiologia do medo e da ansiedade
• Beneficiário: Marcus Lira Brandão
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 06/03930-5 - Inibição da resposta aversiva neurocininérgica na matéria cinzenta periaquedutal dorsal de ratos Wistar, através do bloqueio local de receptores NK1, avaliada pelo teste do labirinto em cruz elevado
• Beneficiário: Gabriel Shimizu Bassi
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica