Effects of Bothrops asper snake venom on the expression of cyclooxygenases and production of prostaglandins by peritoneal leukocytes in vivo, and by isolated neutrophils and macrophages in vitro

Autor(es)

Moreira, Vanessa ^[1] ; Gutierrez, Jose Maria ^[2] ; Amaral, Rafaela Bacci ^[1] ; Zamuner, Stella Regina ^[1] ; Pereira Teixeira, Catarina de Fatima ^[1]

Afiliação do(s) autor(es)

^[1] Inst Butantan, Farmacol Lab, BR-05503900 Sao Paulo - Brazil

^[2] Univ Costa Rica, Fac Microbiol, Inst Clodomiro Picado, San Jose - Costa Rica

• Tipo de documento: Artigo Científico
• Fonte: PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS; v. 80, n. 2-3, p. 107-114, FEB-MAR 2009.
• Citações Web of Science: 9

Resumo

In this study, the ability of Bothrops asper snake venom (BaV) to increase the production of prostaglandins PGE(2) and PGD(2) was assessed in a mouse model in vivo and in inflammatory cells in vitro. In addition, the expressions of COX-1 and COX-2 were assessed. BaV induced an increment in the in vivo synthesis of PGE(2) and PGD(2), together with an enhanced expression of COX-2, but not of COX-1 However, enzymatic activities of COX-1 and COX-2 were increased. Incubation of isolated macrophages and neutrophils with a sub-cytotoxic concentration of BaV in vitro resulted in increased release of PGE(2) and PGD2, by macrophages and PGE(2) by neutrophils, concomitantly with an increment in the expression of COX-2, but not of COX-1 by both cell types. Our results demonstrate the ability of BaV to promote the expression of COX-2 and to induce the synthesis of proinflammatory prostaglandins. Macrophages and neutrophils may be important targets for this venom under in vivo situation. (C) 2008 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 02/13458-0 - Estudo dos efeitos de toxinas com estrutura de fosfolipase a2 isoladas do veneno de bothrops asper e da crotoxina do veneno de crotalus durissus terrificus sobre a expressao de ciclooxigenases...
• Beneficiário: Vanessa Moreira
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 02/13863-2 - Efeitos dos venenos de Bothrops asper e de Crotalus durissus terrificus, das miotoxinas MT-II e MT-III, crotoxina e crotapotina sobre a liberação de prostanóides e a expressão de ciclooxigenases: estudos in vivo e in vitro
• Beneficiário: Catarina de Fatima Pereira Teixeira
• Linha de fomento: Auxílio à Pesquisa - Regular