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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of Bothrops asper snake venom on the expression of cyclooxygenases and production of prostaglandins by peritoneal leukocytes in vivo, and by isolated neutrophils and macrophages in vitro

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Author(s):
Moreira, Vanessa [1] ; Gutierrez, Jose Maria [2] ; Amaral, Rafaela Bacci [1] ; Zamuner, Stella Regina [1] ; Pereira Teixeira, Catarina de Fatima [1]
Total Authors: 5
Affiliation:
[1] Inst Butantan, Farmacol Lab, BR-05503900 Sao Paulo - Brazil
[2] Univ Costa Rica, Fac Microbiol, Inst Clodomiro Picado, San Jose - Costa Rica
Total Affiliations: 2
Document type: Journal article
Source: PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS; v. 80, n. 2-3, p. 107-114, FEB-MAR 2009.
Web of Science Citations: 9
Abstract

In this study, the ability of Bothrops asper snake venom (BaV) to increase the production of prostaglandins PGE(2) and PGD(2) was assessed in a mouse model in vivo and in inflammatory cells in vitro. In addition, the expressions of COX-1 and COX-2 were assessed. BaV induced an increment in the in vivo synthesis of PGE(2) and PGD(2), together with an enhanced expression of COX-2, but not of COX-1 However, enzymatic activities of COX-1 and COX-2 were increased. Incubation of isolated macrophages and neutrophils with a sub-cytotoxic concentration of BaV in vitro resulted in increased release of PGE(2) and PGD2, by macrophages and PGE(2) by neutrophils, concomitantly with an increment in the expression of COX-2, but not of COX-1 by both cell types. Our results demonstrate the ability of BaV to promote the expression of COX-2 and to induce the synthesis of proinflammatory prostaglandins. Macrophages and neutrophils may be important targets for this venom under in vivo situation. (C) 2008 Elsevier Ltd. All rights reserved. (AU)