Transcriptional changes in U343 MG-a glioblastoma cell line exposed to ionizing radiation

Autor(es):Mostrar menos -

Bassi, C. L. ^[1] ; Mello, S. S. ^[1] ; Cardoso, R. S. ^[1] ; Godoy, P. D. V. ; Fachin, A. L. ^[1] ; Junta, C. M. ^[1] ; Sandrin-Garcia, P. ^[1] ; Carlotti, C. G. ^[2] ; Falcao, R. P. ^[3] ; Donadi, E. A. ^[4] ; Passos, G. A. S. ^{[1, 5]} ; Sakamoto-Hojo, E. T. ^{[6, 1]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Genet, Fac Med FMRP, BR-14040901 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Dept Surg & Anat, Fac Med FMRP, BR-14040901 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Dept Hematol, Fac Med FMRP, BR-14040901 Ribeirao Preto, SP - Brazil

^[4] Univ Sao Paulo, Dept Clin Med, Fac Med FMRP, BR-14040901 Ribeirao Preto, SP - Brazil

^[5] Fac Dent FORP, Dept Morphol, Ribeirao Preto, SP - Brazil

^[6] Univ Sao Paulo, Dept Biol, Fac Philosophy Sci & Letters FFCLRP, BR-14040901 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: HUMAN & EXPERIMENTAL TOXICOLOGY; v. 27, n. 12, p. 919-929, DEC 2008.
• Citações Web of Science: 14

Resumo

Glioblastoma multiforme (GBM) is a highly invasive and radioresistant brain tumor. Aiming to study how glioma cells respond to gamma-rays in terms of biological processes involved in cellular responses, we performed experiments at cellular context and gene expression analysis in U343-MG-a GBM cells irradiated with 1 Gy and collected at 6 h post-irradiation. The survival rate was approximately 61% for 1 Gy and was completely reduced at 16 Gy. By performing the microarray technique, 859 cDNA clones were analyzed. The Significance Analysis of Microarray algorithm indicated 196 significant expressed genes (false discovery rate (FDR) = 0.42%): 67 down-regulated and 97 up-regulated genes, which belong to several classes: metabolism, adhesion/cytoskeleton, signal transduction, cell cycle/apoptosis, membrane transport, DNA repair/DNA damage signaling, transcription factor, intracellular signaling, and RNA processing. Differential expression patterns of five selected genes (HSPA9B, INPP5A, PIP5K1A, FANCG, and TPP2) observed by the microarray analysis were further confirmed by the quantitative real time RT-PCR method, which demonstrated an up-regulation status of those genes. These results indicate a broad spectrum of biological processes (which may reflect the radio-resistance of U343 cells) that were altered in irradiated glioma cells, so as to guarantee cell survival. (AU)

• Processo FAPESP: 99/12135-9 - Projeto transcriptoma: análise da expressão gênica em larga escala usando DNA-arrays
• Beneficiário: Geraldo Aleixo da Silva Passos Júnior
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 04/15611-6 - Mecanismos envolvidos nas respostas celulares aos compostos antitumorais e raios-gama em diferentes tipos de celulas humanas.
• Beneficiário: Elza Tiemi Sakamoto Hojo
• Modalidade de apoio: Auxílio à Pesquisa - Regular