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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Production and characteristics comparison of crude beta-glucosidases produced by microorganisms Thermoascus aurantiacus e Aureobasidium pullulans in agricultural wastes

Texto completo
Autor(es):
Ribeiro Leite, Rodrigo Simoes [1] ; Alves-Prado, Heloiza Ferreira [2] ; Cabral, Hamilton [3] ; Pagnocca, Fernando Carlos [2] ; Gomes, Eleni [1] ; Da-Silva, Roberto [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ, UNESP, IBILCE, Lab Biochem & Appl Microbiol, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil
[2] Sao Paulo State Univ, UNESP, Fac Engn Ilha Solteira, FEIS, BR-15385000 Ilha Solteira, SP - Brazil
[3] Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, Dept Pharmaceut Sci, BR-14040903 Ribeirao Preto, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Enzyme and Microbial Technology; v. 43, n. 6, p. 391-395, NOV 6 2008.
Citações Web of Science: 63
Assunto(s):Enzimas   beta-Glucosidase   Thermoascus   Celulase
Resumo

The purified beta-glucosidase of Aureobasidium pullulans ER-16 is one of more thermostable enzyme reported to date. Considering the unfeasibility of using purified enzyme for industrial application, it was interesting to analyze this property for the crude enzyme. Thermophilic fungus Thermoascus aurantiacus CBMAI-756 and mesophilic A. pullulans ER-16 were cultivated in different hemicellulosic materials on solid-state cultivation for beta-glucosidase production. Wheat bran was most appropriate for beta-glucosidase production by both microorganisms. T. aurantiacus exhibited maximum enzyme production (7.0 U/ml or 70 U/g) at 48-72 h and A. pullulans a maximum (1.3 U/ml or 13 U/g) at 120 h. Maximum activities were at 75 degrees C with optimum pH at 4.5 and 4.0, for T aurantiacus and A. pullulans, respectively. A. pullulans's beta-glucosidase was more pH stable (4.5-10.0 against 4.5-8.0) and more thermostable (90% after 1 h at 75 degrees C against 85% after 1 h at 70 degrees C) than the enzyme from the thermophilic T. aurantiacus. The t((1/2)) at 80 degrees C were 50 and 12.5 min for A. pullulans and T. aurantiascus, respectively. These data confirm the high thermostability of crude beta-glucosidase from A. pullulans. Both beta-glucosidases were strongly inhibited by glucose, but ethanol significantly increased the activity of the enzyme from T. aurantiacus. (C) 2008 Elsevier Inc. All rights reserved. (AU)

Processo FAPESP: 02/05642-6 - Purificação e caracterização bioquímica de beta-glicosidases produzidas pelos microrganismos Aureobasidium sp. e Thermoascus aurantiacus
Beneficiário:Rodrigo Simões Ribeiro Leite
Modalidade de apoio: Bolsas no Brasil - Mestrado
Processo FAPESP: 04/02096-6 - Aplicacao das b-glicosidases produzidas pelos microrganismos aureobasidium pullulans e thermoascus aurantiacus em derivados de soja, sucos e bebidas e estudos cristalograficos das enzimas.
Beneficiário:Rodrigo Simões Ribeiro Leite
Modalidade de apoio: Bolsas no Brasil - Doutorado