Human saphenous vein organ culture under controlled hemodynamic conditions

Autor(es)

Miyakawa, Ayumi Aurea ^[1] ; Oliveira Dallan, Luis Alberto ^[2] ; Lacchini, Silvia ^[2] ; Borin, Thaiz Ferraz ^[2] ; Krieger, Jose Eduardo ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med, Heart Inst InCor, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Med, Dept Med LIM 13, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Clinics; v. 63, n. 5, p. 683-688, OCT 2008.
• Citações Web of Science: 17

Resumo

INTRODUCTION: Saphenous vein grafting is still widely used to revascularize ischemic myocardium. The effectiveness of this procedure is limited by neointima formation and accelerated atherosclerosis, which frequently leads to graft occlusion. A better understanding of this process is important to clarify the mechanisms of vein graft disease and to aid in the formulation of strategies for prevention and/or therapeutics. OBJECTIVE: To develop an ex vivo flow system that allows for controlled hemodynamics in order to mimic arterial and venous conditions. METHODS: Human saphenous veins were cultured either under venous (flow: 5 ml/min) or arterial hemodynamic conditions (flow: 50 ml/min, pressure: 80 mmHg) for 1-, 2- and 4-day periods. Cell viability, cell density and apoptosis were compared before and after these intervals using MTT, Hoeschst 33258 stain, and TUNEL assays, respectively. RESULTS: Fresh excised tissue segments were well preserved prior to the study. Hoechst 33258 and MTT stains showed progressive losses in cell density and cell viability in veins cultured under arterial hemodynamic conditions from 1 to 4 days, while no alterations were observed in veins cultured under venous conditions. Although the cell density from 1-day cultured veins under arterial conditions was similar to that of freshly excised veins, the TUNEL assay indicated that most of these cells were undergoing apoptosis. CONCLUSION: The results observed resemble the events taking place during early in vivo arterial-vein grafting and provide evidence that an ex vivo perfusion system may be useful for the identification of new therapeutic targets that ameliorate vein graft remodeling and increase graft patency over time. (AU)

• Processo FAPESP: 99/11908-4 - Influencia da enzima conversora da angiotensina (eca) nas respostas a injuria vascular.
• Beneficiário: Silvia Lacchini
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 03/01828-0 - Genes diferentemente expressos em modelo de arterialização de enxerto venoso no rato: identificação e caracterização de novos alvos terapêuticos
• Beneficiário: Thaiz Ferraz Borin
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 01/00009-0 - Estudo integrado da hipertensão arterial: caracterização molecular e funcional do sistema cardiovascular
• Beneficiário: Eduardo Moacyr Krieger
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 00/09485-7 - Identificacao de genes com expressao diferenciada em veia safena submetida ao regime arterial.
• Beneficiário: Ayumi Aurea Miyakawa Yamaguchi
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado