Tyrosine Phosphorylation in Nitric Oxide-Mediated Signaling Events

Autor(es)

Monteiro‚ H.P. ; Rocha Oliveira‚ C.J. ; Curcio‚ M.F. ; Moraes‚ M.S. ; Arai‚ R.J.

• Tipo de documento: Artigo Científico
• Fonte: Methods in Enzymology; v. 396, p. 350-358, 2005.

Resumo

In this chapter, we provide an overview of nitric oxide (NO)-tyrosine phosphorylation signal transduction pathways, integrating them with the cyclic guanosine monophosphate (cGMP) and S-nitrosylation-mediated pathways that are triggered by NO. The second half of this chapter includes a description of the methods that our laboratory has used extensively to characterize the mechanisms involved in signaling events mediated by this pathway. These include assays for detecting protein tyrosine phosphorylation, tyrosine phosphorylation of the epidermal growth factor (EGF) receptor, phosphorylation of the ERK1/2 mitogen-activated protein (MAP) kinases, transfection of cells with modified forms of p21Ras, and an assay of p21Ras. (AU)

• Processo FAPESP: 00/12154-2 - Fisiologia molecular da sinalização redox no sistema vascular e modelos de células em cultura
• Beneficiário: Francisco Rafael Martins Laurindo
• Modalidade de apoio: Auxílio à Pesquisa - Temático