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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Interleukin 18 messenger RNA and proIL-18 protein expression in chorioamniotic membranes from pregnant women with preterm prelabor rupture of membranes

Texto completo
Autor(es):
Polettini, Jossimara [1] ; Vieira, Eliane Passarelli ; dos Santos, Mariana Perlati ; Peracoli, Jose Carlos [2] ; Witkin, Steven S. [3] ; da Silva, Marcia Guimaraes [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] UNESP Univ Estadual Paulista, Fac Med Botucatu, Dept Patol, Botucatu Med Sch, BR-18618970 Botucatu, SP - Brazil
[2] UNESP Univ Estadual Paulista, Botucatu Med Sch, Dept Gynecol & Obstet, BR-18618970 Botucatu, SP - Brazil
[3] Cornell Univ, Dept Obstet & Gynecol, Weill Med Coll, New York, NY 10021 - USA
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: EUROPEAN JOURNAL OF OBSTETRICS & GYNECOLOGY AND REPRODUCTIVE BIOLOGY; v. 161, n. 2, p. 134-139, APR 2012.
Citações Web of Science: 3
Resumo

Objective: To quantify the expression of IL-18 mRNA and protein in the chorioamniotic membranes of pregnant women with PPROM and correlate expression with histological chorioamnionitis. Study design: A case control study that included 42 pregnant women not in labor in the following groups: PPROM (n = 28) and controls with intact membranes submitted to selective cesarean section at term (n = 14). Expression of IL-18 mRNA in chorioamniotic membranes was determined by real-time polymerase chain reaction, and IL-18 protein expression was measured by western blot. Histopathological analyses and immunolocalization of IL-18 by immunohistochemistry were also performed. Analyses were performed using the Mann-Whitney or Fisher's exact tests and the group effect was considered significant if the adjusted p-values were <0.05 and the magnitude of change was greater than 2-fold for mRNA expression. Results: IL-18 mRNA was present in 100% of samples and no difference in expression was observed between term vs. PPROM membranes (fold-change 0.12; p = 0.88). In the PPROM group, no difference was observed in IL-18 mRNA regarding gestational age (fold-change 0.11; p = 0.42) or the presence of histological chorioamnionitis (fold-change 0.26; p = 0.15). ProIL-18 was present in all samples. IL-18 was immunolocalized to amnion, chorion and decidua cells, with intense immunohistochemical staining at the choriodecidual junction. Conclusion: Chorioamniotic membranes are sources of IL-18 mRNA and proIL-18, and their expression is unrelated to PPROM or histological chorioamnionitis. (C) 2012 Elsevier Ireland Ltd. All rights reserved. (AU)

Processo FAPESP: 08/55517-0 - Análise da expressão de IL-18 em gestações complicadas por rotura prematura de membranas pré-termo
Beneficiário:Mariana Perlati dos Santos
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 08/55417-5 - Análise da expressão de IL-18 em gestações complicadas por rotura prematura de membranas pré-termo
Beneficiário:Márcia Guimarães da Silva
Linha de fomento: Auxílio à Pesquisa - Regular