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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Interleukin 18 messenger RNA and proIL-18 protein expression in chorioamniotic membranes from pregnant women with preterm prelabor rupture of membranes

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Author(s):
Polettini, Jossimara [1] ; Vieira, Eliane Passarelli ; dos Santos, Mariana Perlati ; Peracoli, Jose Carlos [2] ; Witkin, Steven S. [3] ; da Silva, Marcia Guimaraes [1]
Total Authors: 6
Affiliation:
[1] UNESP Univ Estadual Paulista, Fac Med Botucatu, Dept Patol, Botucatu Med Sch, BR-18618970 Botucatu, SP - Brazil
[2] UNESP Univ Estadual Paulista, Botucatu Med Sch, Dept Gynecol & Obstet, BR-18618970 Botucatu, SP - Brazil
[3] Cornell Univ, Dept Obstet & Gynecol, Weill Med Coll, New York, NY 10021 - USA
Total Affiliations: 3
Document type: Journal article
Source: EUROPEAN JOURNAL OF OBSTETRICS & GYNECOLOGY AND REPRODUCTIVE BIOLOGY; v. 161, n. 2, p. 134-139, APR 2012.
Web of Science Citations: 3
Abstract

Objective: To quantify the expression of IL-18 mRNA and protein in the chorioamniotic membranes of pregnant women with PPROM and correlate expression with histological chorioamnionitis. Study design: A case control study that included 42 pregnant women not in labor in the following groups: PPROM (n = 28) and controls with intact membranes submitted to selective cesarean section at term (n = 14). Expression of IL-18 mRNA in chorioamniotic membranes was determined by real-time polymerase chain reaction, and IL-18 protein expression was measured by western blot. Histopathological analyses and immunolocalization of IL-18 by immunohistochemistry were also performed. Analyses were performed using the Mann-Whitney or Fisher's exact tests and the group effect was considered significant if the adjusted p-values were <0.05 and the magnitude of change was greater than 2-fold for mRNA expression. Results: IL-18 mRNA was present in 100% of samples and no difference in expression was observed between term vs. PPROM membranes (fold-change 0.12; p = 0.88). In the PPROM group, no difference was observed in IL-18 mRNA regarding gestational age (fold-change 0.11; p = 0.42) or the presence of histological chorioamnionitis (fold-change 0.26; p = 0.15). ProIL-18 was present in all samples. IL-18 was immunolocalized to amnion, chorion and decidua cells, with intense immunohistochemical staining at the choriodecidual junction. Conclusion: Chorioamniotic membranes are sources of IL-18 mRNA and proIL-18, and their expression is unrelated to PPROM or histological chorioamnionitis. (C) 2012 Elsevier Ireland Ltd. All rights reserved. (AU)