Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Acute cocaine treatment increases thimet oligopeptidase in the striatum of rat brain

Texto completo
Autor(es):
Mostrar menos -
Dalio, Fernanda M. [1] ; Visniauskas, Bruna [2] ; Bicocchi, Eliane S. [3] ; Perry, Juliana C. [2] ; Freua, Rodrigo [1] ; Gesteira, Tarsis F. [4] ; Nader, Helena B. [4] ; Machado, Mauricio F. M. [1] ; Tufik, Sergio [2] ; Ferro, Emer S. [5] ; Andersen, Monica L. [2] ; Toledo, Claudio A. B. [3] ; Chagas, Jair R. [2, 6] ; Oliveira, Vitor [1]
Número total de Autores: 14
Afiliação do(s) autor(es):
[1] Univ Fed Sao Paulo UNIFESP, Dept Biofis, BR-04039032 Sao Paulo - Brazil
[2] Univ Fed Sao Paulo UNIFESP, Dept Psicobiol, BR-04039032 Sao Paulo - Brazil
[3] Univ Cidade Sao Paulo UNICID, Lab Neurociencias, Sao Paulo - Brazil
[4] Univ Fed Sao Paulo UNIFESP, Dept Bioquim, BR-04039032 Sao Paulo - Brazil
[5] Univ Sao Paulo, Dept Biol Celular & Desenvolvimento, BR-05508 Sao Paulo - Brazil
[6] Univ Fed Sao Paulo UNIFESP, Dept Biociencias, BR-04039032 Sao Paulo - Brazil
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: Biochemical and Biophysical Research Communications; v. 419, n. 4, p. 724-727, MAR 23 2012.
Citações Web of Science: 1
Resumo

Many studies indicate that thimet oligopeptidase (EC3.4.24.15; TOP) can be implicated in the metabolism of bioactive peptides, including dynorphin 1-8, alpha-neoendorphin, beta-neoendorphin and GnRH. Furthermore, the higher levels of this peptidase are found in neuroendocrine tissue and testis. In the present study, we have evaluated the effect of acute cocaine administration in male rats on TOP specific activity and mRNA levels in prosencephalic brain areas related with the reward circuitry; ventral striatum, hippocampus, and frontal cortex. No significant differences on TOP specific activity were detected in the hippocampus and frontal cortex of cocaine treated animals compared to control vehicle group. However, a significant increase in activity was observed in the ventral striatum of cocaine treated-rats. The increase occurred in both, TOP specific activity and TOP relative mRNA amount determined by real time RT-PCR. As TOP can be implicated in the processing of many neuropeptides, and previous studies have shown that cocaine also alters the gene expression of proenkephalin and prodynorphin in the striatum, the present findings suggest that TOP changes in the brain could play important role in the balance of neuropeptide level correlated with cocaine effects. (C) 2012 Elsevier Inc. All rights reserved. (AU)

Processo FAPESP: 98/14303-3 - Center for Sleep Studies
Beneficiário:Sergio Tufik
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs