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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Acute cocaine treatment increases thimet oligopeptidase in the striatum of rat brain

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Author(s):
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Dalio, Fernanda M. [1] ; Visniauskas, Bruna [2] ; Bicocchi, Eliane S. [3] ; Perry, Juliana C. [2] ; Freua, Rodrigo [1] ; Gesteira, Tarsis F. [4] ; Nader, Helena B. [4] ; Machado, Mauricio F. M. [1] ; Tufik, Sergio [2] ; Ferro, Emer S. [5] ; Andersen, Monica L. [2] ; Toledo, Claudio A. B. [3] ; Chagas, Jair R. [2, 6] ; Oliveira, Vitor [1]
Total Authors: 14
Affiliation:
[1] Univ Fed Sao Paulo UNIFESP, Dept Biofis, BR-04039032 Sao Paulo - Brazil
[2] Univ Fed Sao Paulo UNIFESP, Dept Psicobiol, BR-04039032 Sao Paulo - Brazil
[3] Univ Cidade Sao Paulo UNICID, Lab Neurociencias, Sao Paulo - Brazil
[4] Univ Fed Sao Paulo UNIFESP, Dept Bioquim, BR-04039032 Sao Paulo - Brazil
[5] Univ Sao Paulo, Dept Biol Celular & Desenvolvimento, BR-05508 Sao Paulo - Brazil
[6] Univ Fed Sao Paulo UNIFESP, Dept Biociencias, BR-04039032 Sao Paulo - Brazil
Total Affiliations: 6
Document type: Journal article
Source: Biochemical and Biophysical Research Communications; v. 419, n. 4, p. 724-727, MAR 23 2012.
Web of Science Citations: 1
Abstract

Many studies indicate that thimet oligopeptidase (EC3.4.24.15; TOP) can be implicated in the metabolism of bioactive peptides, including dynorphin 1-8, alpha-neoendorphin, beta-neoendorphin and GnRH. Furthermore, the higher levels of this peptidase are found in neuroendocrine tissue and testis. In the present study, we have evaluated the effect of acute cocaine administration in male rats on TOP specific activity and mRNA levels in prosencephalic brain areas related with the reward circuitry; ventral striatum, hippocampus, and frontal cortex. No significant differences on TOP specific activity were detected in the hippocampus and frontal cortex of cocaine treated animals compared to control vehicle group. However, a significant increase in activity was observed in the ventral striatum of cocaine treated-rats. The increase occurred in both, TOP specific activity and TOP relative mRNA amount determined by real time RT-PCR. As TOP can be implicated in the processing of many neuropeptides, and previous studies have shown that cocaine also alters the gene expression of proenkephalin and prodynorphin in the striatum, the present findings suggest that TOP changes in the brain could play important role in the balance of neuropeptide level correlated with cocaine effects. (C) 2012 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 98/14303-3 - Center for Sleep Studies
Grantee:Sergio Tufik
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC