New insights about the posttranscriptional mechanisms triggered by iodide excess on sodium/iodide symporter (NIS) expression in PCCl3 cells

Autor(es)

Serrano-Nascimento, Caroline ^[1] ; Calil-Silveira, Jamile ^[1] ; Goulart-Silva, Francemilson ^[1] ; Nunes, Maria Tereza ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, BR-05508000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Molecular and Cellular Endocrinology; v. 349, n. 2, p. 154-161, FEB 26 2012.
• Citações Web of Science: 9

Resumo

Iodide excess acutely downregulates NIS mRNA expression, as already demonstrated. PCCl3 cells treated or not with Nal, Nal + NaClO4 or Nal + Methimazole, for 30 min to 24 h, were used to further explore how iodide reduces NIS gene expression. NIS mRNA expression was evaluated by Real-Time PCR; its poly(A) tail length, by RACE-PAT; its translation rate, by polysome profile; total NIS content, by Western blotting. NIS mRNA decay rate was evaluated in actinomycin-D-treated cells, incubated with or without Nal for 0-6 h. Iodide treatment caused a reduction in NIS mRNA expression, half-life, poly(A) tail length, recruitment to ribosomes, as well as NIS protein expression. Perchlorate, but not methimazole, prevented these effects. Therefore, reduced poly(A) tail length of NIS mRNA seems to be related to its decreased half-life, in addition to its translation impairment. These data provide new insights about the molecular mechanisms involved in the rapid and posttranscriptional inhibitory effect of iodide on NIS expression. (C) 2011 Elsevier Ireland Ltd. All rights reserved. (AU)

• Processo FAPESP: 08/56446-9 - Analise molecular das acoes pos-transcricionais dos hormonios tiroideanos nas celulas beta pancreaticas e suas repercussoes sobre a sintese e secrecao de insulina
• Beneficiário: Francemilson Goulart da Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 10/05503-2 - Efeito da administração aguda de iodo sobre a expressão e a atividade do promotor do gene da pendrina: estudo in vivo e in vitro
• Beneficiário: Jamile Calil Silveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 09/17834-6 - Estudo das bases moleculares envolvidas na regulação a curto prazo da expressão gênica do co-transportador Na+/I- e pendrina pelo iodo em tireóide de ratos e células PCCl3: possível contribuição para a compreensão do efeito Wolff-Chaikoff e seu escape
• Beneficiário: Maria Tereza Nunes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/50175-6 - Bases moleculares envolvidas na regulacao da expressao do gene do co-transportador de sodio-iodeto (nis) pelo iodeto.
• Beneficiário: Caroline Serrano Do Nascimento
• Modalidade de apoio: Bolsas no Brasil - Doutorado