Local inflammatory events induced by Bothrops atrox snake venom and the release of distinct classes of inflammatory mediators

Autor(es)

Moreira, Vanessa ^[1] ; Dos-Santos, Maria Cristina ^[2] ; Nascimento, Neide Galvao ^[1] ; da Silva, Henrique Borges ^[3] ; Fernandes, Cristina Maria ^[1] ; D'Imperio Lima, Maria Regina ^[3] ; Teixeira, Catarina ^[1]

Afiliação do(s) autor(es)

^[1] Inst Butantan, Farmacol Lab, BR-05503900 Sao Paulo - Brazil

^[2] Univ Fed Amazonas, Dept Parasitol, ICB, Lab Imunol, Manaus, AM - Brazil

^[3] Univ Sao Paulo, Inst Ciencias Biomed, Dept Imunol, BR-05508 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Toxicon; v. 60, n. 1, p. 12-20, JUL 2012.
• Citações Web of Science: 29

Resumo

Bothrops atrox is responsible for most accidents involving snakes in the Brazilian Amazon and its venom induces serious systemic and local effects. The local effects are not neutralized effectively by commercial antivenoms, resulting in serious sequelae in individuals bitten by this species. This study investigates the local inflammatory events induced in mice by B. atrox venom (Bay), such as vascular permeability, leukocyte influx and the release of important inflammatory mediators such as cytokines, eicosanoids and the chemokine CCL-2, at the injection site. The effect of Bay on cyclooxygenase (COX-1 and COX-2) expression was also investigated. The results showed that intraperitoneal (i.p.) injection of BaV promoted a rapid and significant increase in vascular permeability, which reached a peak 1 h after venom administration. Furthermore, BaV caused leukocyte infiltration into the peritoneal cavity between 1 and 8 h after i.p. injection, with mononuclear leukocytes (MNs) predominating in the first 4 h, and polymorphonuclear leukocytes (PMNs) in the last 4 h. Increased protein expression of COX-2, but not of COX-1, was detected in leukocytes recruited in the first and fourth hours after injection of BaV. The venom caused the release of eicosanoids PGD(2), PGE(2), TXA(2) and LTB4, cytokines TNF-alpha, IL-6, IL-10 and IL-12p70, but not IFN-gamma, and chemokine CCL-2 at different times. The results show that Bay is able to induce an early increase in vascular permeability and a leukocyte influx to the injection site consisting mainly of MNs initially and PMNs during the later stages. These phenomena are associated with the production of cytokines, the chemokine CCL-2 and eicosanoids derived from COX-1 and COX-2. (c) 2012 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 10/51150-4 - Modelo animal para análise da patogenicidade de cepas de Mycobacterium bovis e avaliação da resposta imune celular e humoral contra isolados patogênicos
• Beneficiário: Maria Regina D'Império Lima
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 07/03336-9 - Estudo de efeitos de duas fosfolipases A2, isoladas do veneno de serpente, sobre vias de ativação do NF-kB relacionados à expressão de COX-2 e de PGE sintase M-1: envolvimento do receptor de manose
• Beneficiário: Catarina de Fatima Pereira Teixeira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/08559-1 - Caracterização dos mecanismos efetores da imunidade inata e adquirida no modelo de malária crônica em camundongos CD28KO infectados pelo Plasmodium chabaudi AS
• Beneficiário: Henrique Borges da Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 07/03337-5 - Efeito de duas fosfolipases A2, isoladas do veneno de serpente, sobre vias de ativação do NF-kapa b relacionadas à expressão de COX-2 e de PGE sintase m-1: envolvimento do receptor de manose de macrófagos nesses eventos
• Beneficiário: Vanessa Moreira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado