RHD Allelic Identification Among D-Brazilian Blood Donors as a Routine Test Using Pools of DNA

Autor(es)

Mota, Mariza ^[1] ; Dezan, M. ^[1] ; Valgueiro, M. C. ^[2] ; Sakashita, A. M. ^[1] ; Kutner, J. M. ^[1] ; Castilho, L. ^[3]

Afiliação do(s) autor(es)

^[1] Albert Einstein Hosp, Dept Hemotherapy, BR-05652000 Sao Paulo - Brazil

^[2] HEMOPE, Blood Bank, Recife, PE - Brazil

^[3] Hemoctr UNICAMP, INCTs, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF CLINICAL LABORATORY ANALYSIS; v. 26, n. 2, p. 104-108, 2012.
• Citações Web of Science: 10

Resumo

Background: RHD alleles leading to a reduced expression of D antigen of the red blood cell (RBC) surface may be erroneously typed as D- by serology and may cause anti-D immunizations when transfused to recipients. Methods: To determine the occurrence of such alleles among apparent D- blood donors, molecular typing was implemented as a routine test using a pool of DNA. A total of 2,450 pretyped D- samples were tested in pools of 10 for the RHD-specific polymorphism in intron 4 and exon 7. Samples in polymer chain reaction (PCR) positive pools were individually reevaluated by exon-specific PCRs, sequencing, and serologic methods. Results: Among 2,450 serologically D- blood donor samples tested, 101 (4.1%) carried the RHD gene. Nonfunctional RHD (RHD psi, RHD{*}CE(2-9)-D, and RHD{*}CE(37)- D), different weak D alleles such as RHD{*}weak D type 1, RHD{*}weak D type 4.3, RHD{*}weak D type 5, RHD{*}weak D type 38, and RHD{*}DEL were identified. Conclusion: We employed a PCR-based assay for RHD as a routine test using pools of ten DNA blood donor samples. The integration of RHD genotyping into the routine screening program using pools of DNA samples was straightforward. As a consequence, 19 (0.8%) blood donors carrying a weak D and Del phenotypes with the potential of causing anti-D immunizations in recipients were reclassified as D+. For each population, it would be necessary to adapt the RHD genotyping strategy to the spectrum of prevalent alleles. J. Clin. Lab. Anal. 26:104-108, 2012. (C) 2012 Wiley Periodicals, Inc. (AU)

• Processo FAPESP: 09/05924-0 - Análise de polimorfismos e expressão de antígenos de grupos sanguíneos: implicações clínicas
• Beneficiário: Lilian Maria de Castilho
• Modalidade de apoio: Auxílio à Pesquisa - Regular