Fhos encodes a Drosophila Formin-Like Protein participating in autophagic programmed cell death

Autor(es)

Anhezini, Lucas ^[1] ; Saita, Ana Paula ^[1] ; Costa, Mara S. A. ^[1] ; Pinheiro Ramos, Ricardo Guelerman ^[1] ; Simon, Claudio Roberto ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Med, Dept Cell & Mol Biol & Pathogen Bioagents, Sao Paulo - Brazil

^[2] Univ Fed Triangulo Mineiro, Dept Biol Struct, BR-38025015 Uberaba, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: GENESIS; v. 50, n. 9, p. 672-684, SEP 2012.
• Citações Web of Science: 6

Resumo

Larval tissues undergo programmed cell death (PCD) during Drosophila metamorphosis. PCD is triggered in a stage and tissue-specific fashion in response to ecdysone pulses. The understanding of how ecdysone induces the stage and tissue-specificity of cell death remains obscure. Several steroid-regulated primary response genes have been shown to act as key regulators of cellular responses to ecdysone by inducing a cascade of transcriptional regulation of late responsive genes. In this article, the authors identify Fhos as a gene that is required for Drosophila larval salivary gland destruction. Animals with a P-element mutation in Fhos possess persistent larval salivary glands, and precise excisions of this P-element insertion resulted in reversion of this salivary gland mutant phenotype. Fhos encodes the Drosophila homolog of mammalian Formin Fhos. Fhos is differentially transcribed during development and responds to ecdysone in a method that is similar to other cell death genes. Similarly to what has been shown for its mammalian counterpart, FHOS protein is translocated to the nucleus at later stages of cell death. Fhos mutants posses disrupted actin cytoskeleton dynamics in persistent salivary glands. Together, our data indicate that Fhos is a new ecdysone-regulated gene that is crucial for changes in the actin cytoskeleton during salivary gland elimination in Drosophila. genesis 50:672684, 2012. (c) 2012 Wiley Periodicals, Inc. (AU)

• Processo FAPESP: 09/50208-1 - Analise funcional da proteina jazigo: uma nova formina envolvida na morte celular programada da glandula salivar larval de drosophila melanogaster
• Beneficiário: Ana Paula Saita
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 07/59879-0 - Caracterizacao funcional de jazigo: uma nova proteina formina que participa da morte celular programada durante o desenvolvimento de drosophila melanogaster.
• Beneficiário: Lucas Anhezini de Araujo
• Modalidade de apoio: Bolsas no Brasil - Doutorado