Leucine Supplementation Augments Insulin Secretion in Pancreatic Islets of Malnourished Mice

Autor(es)

Amaral, Andressa G. ^[1] ; Rafacho, Alex ^{[1, 2]} ; de Oliveira, Camila A. Machado ^[1] ; Batista, Thiago M. ^[1] ; Ribeiro, Rosane A. ^[1] ; Latorraca, Marcia Q. ^[3] ; Boschero, Antonio C. ^[1] ; Carneiro, Everardo M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Dept Anat Cell Biol & Physiol & Biophys, Inst Biol, Campinas, SP - Brazil

^[2] Sao Paulo State Univ, Dept Phys Educ, Sch Sci, Sao Paulo - Brazil

^[3] Fed Univ Mato Grosso UFMT, Dept Food & Nutr, Sch Nutr, Cuiaba, Mato Grosso - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PANCREAS; v. 39, n. 6, p. 847-855, AUG 2010.
• Citações Web of Science: 13

Resumo

Objectives: We investigated the influence of leucine supplementation on insulin secretion and on some proteins related to insulin secretion in malnourished mice. Methods: Swiss mice (aged 21 days) received isocaloric normo-17% (NP) or 6% low-protein (LP) diet for 120 days. Half of the NP and LP mice received 1.5% leucine in the drinking water during the last 30 days (NPL and LPL, respectively). Results: The LP mice were hypoinsulinemic compared with the NP group, whereas LPL mice exhibited increased insulinemia in the fed state versus LP mice. The LP mouse islets were less responsive to 22.2 mM glucose, 100 KM carbachol (Cch), and 10 mM leucine than the NP group. However, LPL islets were more responsive to all these conditions compared with the LP group. The muscarinic type 3 receptor, (M3R) CaA2, and PKC-alpha protein contents were reduced in LP compared with NP islets but significantly higher in LPL than LP islets. The p-AKT/AKT ratio was higher in LPL compared with LP islets. Conclusions: Leucine supplementation increases insulin secretion in response to glucose and leucine and to agents that potentiate secretion, such as Cch, in malnourished mice. The enhanced levels of M3R, CaA2, and PKC-alpha proteins, as well as of the p-AKT/AKT ratio, may play a role in this process. (AU)

• Processo FAPESP: 07/50365-4 - Estudo dos mecanismos de destruição das células beta pancreáticas durante a instalação do Diabetes Mellitus (DM2): busca de estratégias para a inibição desse processo bem como para a recuperação da massa insular em diferentes modelos animais
• Beneficiário: Antonio Carlos Boschiero
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 08/53811-8 - Caracterização morfofuncional de ilhotas pancreáticas de camundongos submetidos à restrição protéica ou à dieta hiperlipídica e suplementados com taurina
• Beneficiário: Everardo Magalhães Carneiro
• Modalidade de apoio: Auxílio à Pesquisa - Regular