| Texto completo | |
| Autor(es): |
Machado, M. F. M.
;
Cunha, F. M.
;
Berti, D. A.
;
Heimann, A. S.
;
Klitzke, C. F.
;
Rioli, V.
;
Oliveira, V.
;
Ferro, E. S.
[8]
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Biochemical and Biophysical Research Communications; v. 339, n. 2, p. 518-523, Jan. 2006. |
| Área do conhecimento: | Ciências Biológicas - Morfologia |
| Assunto(s): | Inibidores da enzima conversora da angiotensina Peptídeo hidrolases |
| Resumo | |
Recent findings from our laboratory suggest that intracellular peptides containing putative post-translational modification sites (i.e., phosphorylation) could regulate specific protein interactions. Here, we extend our previous observations showing that peptide phosphorylation changes the kinetic parameters of structurally related endopeptidase EP24.15 (EC 3.4.24.15), neurolysin (EC 3.4.24.16), and angiotensin-converting enzyme (EC 3.4.15.1). Phosphorylation of peptides that are degraded by these enzymes leads to reduced degradation, whereas phosphorylation of peptides that interacted as competitive inhibitors of these enzymes alters only the K-i's. These data suggest that substrate phosphorylation could be one of the mechanisms whereby some intracellular peptides would escape degradation and could be regulating protein interactions within cells. (AU) | |
| Processo FAPESP: | 04/04933-2 - Biologia celular molecular de oligopeptidases |
| Beneficiário: | Emer Suavinho Ferro |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 03/13350-8 - Especificidade e mecanismo de catálise das oligopeptidases neurolisina, timet oligopeptidase e neprilisina: estudos de cinética enzimática associados a mutações sítio-dirigidas |
| Beneficiário: | Vitor Marcelo Silveira Bueno Brandão de Oliveira |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |