Antioxidant defenses and biochemical changes in the neotropical fish pacu, Piaractus mesopotamicus: Responses to single and combined copper and hypercarbia exposure

Autor(es)

Sampaio, Fernanda Garcia ^[1] ; Boijink, Cheila de Lima ^[2] ; Bichara dos Santos, Laila Romagueira ^[3] ; Oba, Eliane Tie ^[4] ; Kalinin, Ana Lucia ^{[1, 5]} ; Barreto Luiz, Alfredo Jose ^[1] ; Rantin, Francisco Tadeu ^{[1, 5]}

Afiliação do(s) autor(es)

^[1] Brazilian Agr Res Corp, Minist Agr Livestock & Food Supply, EMBRAPA, Environm, Jaguariuna, SP - Brazil

^[2] Brazilian Agr Res Corp, Minist Agr Livestock & Food Supply, EMBRAPA, W Amazon, Manaus, Amazonas - Brazil

^[3] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, BR-05508 Sao Paulo - Brazil

^[4] Brazilian Agr Res Corp, Minist Agr Livestock & Food Supply, EMBRAPA, Amapa, Macapa, AP - Brazil

^[5] Natl Inst Sci & Technol, Comparat Physiol FAPESP CNPq, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY; v. 156, n. 3-4, p. 178-186, NOV 2012.
• Citações Web of Science: 8

Resumo

This study investigated the potentially detrimental effects of copper and elevated aquatic CO2 (hypercarbia), alone or in combination, on pacu, Piaractus mesopotamicus. Fish were exposed for 48 h to control (no copper addition in normocarbia), to 400 mu g Cu2+L-1, to hypercarbic (1% CO2; PCO2=6.9 mm Hg) water and to 400 mu g Cu2+L-1+ hypercarbia. In liver the single factors caused an increase in lipid hydroperoxide concentration that was not observed when the factors were combined. Copper exposure elicited increased hepatic superoxide dismutase activity, irrespective of aquatic CO2 level. On the other hand, the effects of copper on hepatic glutathione peroxidase activity were dependent on water CO2 levels. The two stressors combined did not affect hepatic catalase activity. Hypercarbic water caused a decline in plasma glucose concentration, but this was not observed when hypercarbia was combined with copper exposure. Copper caused a decrease in branchial Na+/K+-ATPase activity that was independent of water CO2 level. Copper caused an increase in branchial metallothionein concentration that was independent of water CO2 level. Thus, branchial metallothionein and Na+/K+-ATPase were effective biomarkers of copper exposure that were not affected by water CO2 level. (C) 2012 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 03/06105-7 - Acao pro e antioxidante do cobre: estresse oxidativo e defesas antioxidantes em peixes tropicais.
• Beneficiário: Francisco Tadeu Rantin
• Modalidade de apoio: Auxílio à Pesquisa - Regular