Chemopreventive activity of compounds extracted from Casearia sylvestris (Salicaceae) Sw against DNA damage induced by particulate matter emitted by sugarcane burning near Araraquara, Brazil

Autor(es):Mostrar menos -

Prieto, A. M. ^[1] ; Santos, A. G. ^[2] ; Csipak, A. R. ^[1] ; Caliri, C. M. ^[1] ; Silva, I. C. ^[1] ; Arbex, M. A. ^[3] ; Silva, F. S. ^[4] ; Marchi, M. R. R. ^[4] ; Cavalheiro, A. J. ^[5] ; Silva, D. H. S. ^[5] ; Bolzani, V. S. ^[5] ; Soares, C. P. ^[1]

Afiliação do(s) autor(es)

^[1] UNESP Univ Estadual Paulista, Coll Pharmaceut Sci, Dept Clin Anal, Araraquara - Brazil

^[2] UNESP Univ Estadual Paulista, Coll Pharmaceut Sci, Dept Nat Principles & Toxicol, Araraquara - Brazil

^[3] UNIFESP Fed Univ Sao Paulo, Paulista Coll Med, Dept Internal Med, Sao Paulo - Brazil

^[4] UNESP Univ Estadual Paulista, Inst Chem, Dept Analyt Chem, Araraquara - Brazil

^[5] UNESP Univ Estadual Paulista, Inst Chem, Dept Organ Chem, Araraquara - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Toxicology and Applied Pharmacology; v. 265, n. 3, p. 368-372, DEC 15 2012.
• Citações Web of Science: 5

Resumo

Ethanolic extract of Casearia sylvestris is thought to be antimutagenic. In this study, we attempted to determine whether this extract and casearin X (a clerodane diterpene from C. sylvestris) are protective against the harmful effects of airborne pollutants from sugarcane burning. To that end, we used the Tradescantia micronucleus test in meiotic pollen cells of Tradescantia pallida, the micronucleus test in mouse bone marrow cells, and the comet assay in mouse blood cells. The mutagenic compound was total suspended particulate (TSP) from air. For the Tradescantia micronucleus test, T. pallida cuttings were treated with the extract at 0.13, 0.25, or 0.50 mg/ml. Subsequently, TSP was added at 0.3 mg/ml, and tetrads from the inflorescences were examined for micronuclei. For the micronucleus test in mouse bone marrow cells and the comet assay in mouse blood cells, Balb/c mice were treated for 15 days with the extract-3.9, 7.5, or 15.0 mg/kg body weight (BW)-or with casearin X-03, 0.25, or 12 mg/kg BW after which they received TSP (3.75 mg/kg BW). In T. pallida and mouse bone marrow cells, the extract was antimutagenic at all concentrations tested. In mouse blood cells, the extract was antigenotoxic at all concentrations, whereas casearin X was not antimutagenic but was antigenotoxic at all concentrations. We conclude that C. sylvestris ethanolic extract and casearin X protect DNA from damage induced by airborne pollutants from sugarcane burning. (C) 2012 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 03/02176-7 - Conservação e uso sustentável da diversidade do Cerrado e da Mata Atlântica: diversidade química e prospecção de medicamentos potenciais - fase II
• Beneficiário: Vanderlan da Silva Bolzani
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 06/50892-1 - Avaliação do efeito antimutagênico do extrato etanólico de Casearia sylvestris em relação ao efeito mutagênico de compostos orgânicos e particulados totais de queima de cana-de-açúcar
• Beneficiário: Camila Milva Caliri
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 04/07932-7 - Busca de compostos naturais antitumorais, antioxidantes, antiinflamatórios, antidiabéticos, inibidores de acetilcolinesterase e mieloperoxidase no Cerrado e na Mata Atlântica
• Beneficiário: Dulce Helena Siqueira Silva
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 05/58472-9 - Avaliação do efeito antimutagênico do extrato etanólico de Casearia sylvestris através do ensaio de micronúcleo em células sanguíneas de camundongo e células de inflorescência de Tradescantia pallida
• Beneficiário: Aline Miranda de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica