| Full text | |
| Author(s): Show less - |
Prieto, A. M.
[1]
;
Santos, A. G.
[2]
;
Csipak, A. R.
[1]
;
Caliri, C. M.
[1]
;
Silva, I. C.
[1]
;
Arbex, M. A.
[3]
;
Silva, F. S.
[4]
;
Marchi, M. R. R.
[4]
;
Cavalheiro, A. J.
[5]
;
Silva, D. H. S.
[5]
;
Bolzani, V. S.
[5]
;
Soares, C. P.
[1]
Total Authors: 12
|
| Affiliation: | [1] UNESP Univ Estadual Paulista, Coll Pharmaceut Sci, Dept Clin Anal, Araraquara - Brazil
[2] UNESP Univ Estadual Paulista, Coll Pharmaceut Sci, Dept Nat Principles & Toxicol, Araraquara - Brazil
[3] UNIFESP Fed Univ Sao Paulo, Paulista Coll Med, Dept Internal Med, Sao Paulo - Brazil
[4] UNESP Univ Estadual Paulista, Inst Chem, Dept Analyt Chem, Araraquara - Brazil
[5] UNESP Univ Estadual Paulista, Inst Chem, Dept Organ Chem, Araraquara - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | Toxicology and Applied Pharmacology; v. 265, n. 3, p. 368-372, DEC 15 2012. |
| Web of Science Citations: | 5 |
| Abstract | |
Ethanolic extract of Casearia sylvestris is thought to be antimutagenic. In this study, we attempted to determine whether this extract and casearin X (a clerodane diterpene from C. sylvestris) are protective against the harmful effects of airborne pollutants from sugarcane burning. To that end, we used the Tradescantia micronucleus test in meiotic pollen cells of Tradescantia pallida, the micronucleus test in mouse bone marrow cells, and the comet assay in mouse blood cells. The mutagenic compound was total suspended particulate (TSP) from air. For the Tradescantia micronucleus test, T. pallida cuttings were treated with the extract at 0.13, 0.25, or 0.50 mg/ml. Subsequently, TSP was added at 0.3 mg/ml, and tetrads from the inflorescences were examined for micronuclei. For the micronucleus test in mouse bone marrow cells and the comet assay in mouse blood cells, Balb/c mice were treated for 15 days with the extract-3.9, 7.5, or 15.0 mg/kg body weight (BW)-or with casearin X-03, 0.25, or 12 mg/kg BW after which they received TSP (3.75 mg/kg BW). In T. pallida and mouse bone marrow cells, the extract was antimutagenic at all concentrations tested. In mouse blood cells, the extract was antigenotoxic at all concentrations, whereas casearin X was not antimutagenic but was antigenotoxic at all concentrations. We conclude that C. sylvestris ethanolic extract and casearin X protect DNA from damage induced by airborne pollutants from sugarcane burning. (C) 2012 Elsevier Inc. All rights reserved. (AU) | |
| FAPESP's process: | 03/02176-7 - Conservation and sustainable use of the diversity from Cerrado and Atlantic Forest: chemical diversity and prospecting for potential drugs - phase II |
| Grantee: | Vanderlan da Silva Bolzani |
| Support Opportunities: | BIOTA-FAPESP Program - Thematic Grants |
| FAPESP's process: | 04/07932-7 - Search for potential antitumoral, antioxidant, antiinflammatory, antidiabetic, acetylcholinesterase and mieloperoxidase inhibitory natural compounds from Cerrado and Atlantic Forest |
| Grantee: | Dulce Helena Siqueira Silva |
| Support Opportunities: | BIOTA-FAPESP Program - Thematic Grants |