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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Local Neurotoxicity and Myotoxicity Evaluation of Cyclodextrin Complexes of Bupivacaine and Ropivacaine

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Autor(es):
Saia Cereda, Cintia Maria [1] ; Tofoli, Giovana Radomille [2] ; Maturana, Luiz Gabriel [3] ; Pierucci, Amauri [3] ; Soares Nunes, Lazaro Alessandro [1] ; Franz-Montan, Michelle [1] ; Rodrigues de Oliveira, Alexandre Leite [4] ; Arana, Sarah [5] ; de Araujo, Daniele Ribeiro [6] ; de Paula, Eneida [1]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Dept Biochem, Inst Biol, Campinas, SP - Brazil
[2] Univ Sao Francisco, Lab Mol Biol & Pharmacol, BR-12900000 Braganca Paulista, SP - Brazil
[3] Fed Univ Jequitinhonha & Mucuri Valleys, Dept Basic Sci, Diamantina, MG - Brazil
[4] Univ Estadual Campinas, Dept Anat Celular Biol Physiol & Biophys, Inst Biol, Campinas, SP - Brazil
[5] Univ Estadual Campinas, Dept Histol & Embryol, Inst Biol, Campinas, SP - Brazil
[6] Fed Univ ABC, Human & Nat Sci Ctr, Santo Andre, SP - Brazil
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: ANESTHESIA AND ANALGESIA; v. 115, n. 5, p. 1234-1241, NOV 2012.
Citações Web of Science: 23
Resumo

BACKGROUND: Bupivacaine (BVC) and ropivacaine (RVC) are local anesthetics widely used in surgical procedures. In previous studies, inclusion complexes of BVC or RVC in hydroxypropyl-beta-cyclodextrin (HP-beta-CD) increased differential nervous blockade, compared to the plain anesthetic solutions. In this study we evaluated the local neural and muscular toxicity of these new formulations containing 0.5% BVC or RVC complexed with HP-beta-CD (BVCHP-beta-CD and RVCHP-beta-CD). METHODS: Schwann cell viability was assessed by determination of mitochondrial dehydrogenese activity, and histopathological evaluation of the rat sciatic nerve was used to identify local neurotoxic effects (48 hours and 7 days after the treatments). Evaluations of serum creatine kinase levels and the histopathology of rat gastrocnemius muscle (48 hours after treatment) were also performed. RESULTS: Schwann cell toxicity evaluations revealed no significant differences between complexed and plain local anesthetic formulations. However, use of the complexed local anesthetics reduced serum creatine kinase levels 5.5-fold, relative to the plain formulations. The differences were significant at P < 0.05 (BVC) and P < 0.01 (RVC). The histopathological muscle evaluation showed that differences between groups treated with local anesthetics (BVC or RVC) and their respective complexed formulations (BVCHP-beta-CD or RVCHP-beta-CD) were significant (P < 0.05). CONCLUSIONS: We concluded that the new formulations presented a lower myotoxicity and a similar cytotoxic effect when compared to plain local anesthetic solutions. (Anesth Analg 2012;115:1234-41) (AU)

Processo FAPESP: 07/55189-0 - Avaliação da citotoxidade e da neurotoxicidade local de anestésicos locais em formulações de liberação prolongada
Beneficiário:Cíntia Maria Saia Cereda
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 06/00121-9 - Novas formulações de anestésicos locais de liberação controlada: do desenvolvimento ao teste clínico odontológico
Beneficiário:Eneida de Paula
Linha de fomento: Auxílio à Pesquisa - Temático