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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Kinin-B2 receptor expression and activity during differentiation of embryonic rat neurospheres

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Martins, Antonio H. ; Alves, Janaína M. ; Trujillo, Cleber A. ; Schwindt, Telma T. ; Barnabé, Gabriela F. ; Motta, Fabiana L. T. ; Guimarães, Alessander O. ; Casarini, Dulce E. ; Mello, Luiz E. ; Pesquero, João B. ; Ulrich, Henning [11]
Número total de Autores: 11
Tipo de documento: Artigo Científico
Fonte: Cytometry Part A; v. 73, n. 4, p. 361-368, Apr. 2008.
Área do conhecimento: Ciências Biológicas - Biofísica
Assunto(s):Sistema calicreína-cinina   Cininas
Resumo

Neural progenitor cells were isolated from rat fetal telencephalon and proliferate as neurospheres in the presence of EGF, FGF-2, and heparin. In the absence of these growth factors, neurospheres differentiate into neurons, astrocytes, and oligodendrocytes. Using an embryonal carcinoma cell line as in vitro differentiation model, we have already demonstrated the presence of an autocrine loop system between kinin-B2 receptor activity and secretion of its ligand bradykinin (BK) as prerequisites for final neuronal differentiation (Martins et al., J Biol Chem 2005; 280: 19576-19586). The aim of this study was to verify the activity of the kallikrein-kinin system (KKS) during neural progenitor cell differentiation. Immunofluorescence studies and flow cytometry analysis revealed increases in glial fibrillary acidic protein and -3 tubulin expression and decrease in the number of nestin-positive cells along neurospheres differentiation, indicating the transition of neural progenitor cells to astrocytes and neurons. Kinin-B2 receptor expression and activity, secretion of BK into the medium, and presence of high-molecular weight kininogen suggest the participation of the KKS in neurosphere differentiation. Functional kinin-B2 receptors and BK secretion indicate an autocrine loop during neurosphere differentiation to neurons, astrocytes, and oligodendrocytes, reflecting events occurring during early brain development. (AU)

Processo FAPESP: 01/08827-4 - Modulação artificial da diferenciação neuronal e função de receptores por oligonucleotídeos sintéticos atuantes aos níveis gênico e protéico
Beneficiário:Alexander Henning Ulrich
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
Processo FAPESP: 06/61285-9 - Bases moleculares da diferenciação de células-tronco e progenitoras neurais
Beneficiário:Alexander Henning Ulrich
Linha de fomento: Auxílio à Pesquisa - Temático