Calcium binding to S. mutans grown in the presence or absence of sucrose

Autor(es)

Leitao, Tarcisio Jorge ^[1] ; Andalo Tenuta, Livia Maria ^[1] ; Ishi, Guilherme ^[1] ; Cury, Jaime Aparecido ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Dept Physiol Sci, Piracicaba Dent Sch, Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Oral Research; v. 26, n. 2, p. 100-105, MAR-APR 2012.
• Citações Web of Science: 2

Resumo

Sucrose is the most cariogenic dietary carbohydrate because it is a substrate for insoluble extracellular polysaccharide (IEPS) production in dental biofilms, which can proportionally decrease bacterial density and, consequently, the number of biofilm calcium (Ca) binding sites. Ca bound to bacterial cell walls can be released into the biofilm fluid during a cariogenic challenge, reducing the driving force for mineral dissolution provoked by the pH drop. Thus, we investigated the effect of an IEPS-rich extracellular matrix on bacterial Ca binding after treatment with Ca solutions. Streptococcus mutans Ingbritt 1600 was cultivated in culture broths supplemented with 1.0% sucrose or 0.5% glucose + 0.5% fructose. The IEPS concentration in bacterial pellets was determined after alkaline extraction. Bacterial pellets were treated with 1 mM or 10 mM Ca++ solutions at 37 degrees C for 10 to 60 min. Ca binding to bacterial pellets, determined after acid extraction using the Arsenazo III reagent, was fast and concentration dependent. Although the IEPS concentration was approximately ten times higher in bacterial pellets cultivated in sucrose as compared to its monossaccharides, bound Ca concentration after Ca treatment was similar in both conditions. These results suggest that IEPS may not influence the amount of Ca bound to reservoirs of dental biofilms. (AU)

• Processo FAPESP: 08/00527-0 - Estudo in vitro da cinética de ligação de cálcio em S. mutans cultivados na presença ou ausência de sacarose
• Beneficiário: Guilherme Ishi
• Linha de fomento: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 09/12907-5 - Estudo da retenção do fluoreto no biofilme dental: importância da ligação a íons cálcio na superfície bacteriana
• Beneficiário: Tarcísio Jorge Leitão de Oliveira
• Linha de fomento: Bolsas no Brasil - Mestrado