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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Calcium binding to S. mutans grown in the presence or absence of sucrose

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Author(s):
Leitao, Tarcisio Jorge [1] ; Andalo Tenuta, Livia Maria [1] ; Ishi, Guilherme [1] ; Cury, Jaime Aparecido [1]
Total Authors: 4
Affiliation:
[1] Univ Estadual Campinas, Dept Physiol Sci, Piracicaba Dent Sch, Piracicaba, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Brazilian Oral Research; v. 26, n. 2, p. 100-105, MAR-APR 2012.
Web of Science Citations: 2
Abstract

Sucrose is the most cariogenic dietary carbohydrate because it is a substrate for insoluble extracellular polysaccharide (IEPS) production in dental biofilms, which can proportionally decrease bacterial density and, consequently, the number of biofilm calcium (Ca) binding sites. Ca bound to bacterial cell walls can be released into the biofilm fluid during a cariogenic challenge, reducing the driving force for mineral dissolution provoked by the pH drop. Thus, we investigated the effect of an IEPS-rich extracellular matrix on bacterial Ca binding after treatment with Ca solutions. Streptococcus mutans Ingbritt 1600 was cultivated in culture broths supplemented with 1.0% sucrose or 0.5% glucose + 0.5% fructose. The IEPS concentration in bacterial pellets was determined after alkaline extraction. Bacterial pellets were treated with 1 mM or 10 mM Ca++ solutions at 37 degrees C for 10 to 60 min. Ca binding to bacterial pellets, determined after acid extraction using the Arsenazo III reagent, was fast and concentration dependent. Although the IEPS concentration was approximately ten times higher in bacterial pellets cultivated in sucrose as compared to its monossaccharides, bound Ca concentration after Ca treatment was similar in both conditions. These results suggest that IEPS may not influence the amount of Ca bound to reservoirs of dental biofilms. (AU)

FAPESP's process: 09/12907-5 - Fluoride retention in dental biofilm: role of bacterial calcium binding
Grantee:Tarcísio Jorge Leitão de Oliveira
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 08/00527-0 - In vitro study of the kinetics of Ca-binding in S. mutans grown in the presence of sucrose
Grantee:Guilherme Ishi
Support Opportunities: Scholarships in Brazil - Scientific Initiation