Isolation of natural red colorants from fermented broth using ionic liquid-based aqueous two-phase systems

Autor(es)

Ventura, Sonia P. M. ^[1] ; Santos-Ebinuma, Valeria C. ^[2] ; Pereira, Jorge F. B. ^[1] ; Teixeira, Maria F. S. ^[3] ; Pessoa, Adalberto ^[2] ; Coutinho, Joao A. P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Aveiro, Dept Quim, CICECO, P-3810193 Aveiro - Portugal

^[2] Univ Sao Paulo, Dept Tecnol Bioquim Farmaceut, BR-05508000 Sao Paulo - Brazil

^[3] Univ Fed Amazonas, BR-69077000 Manaus, Amazonas - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Industrial Microbiology & Biotechnology; v. 40, n. 5, p. 507-516, MAY 2013.
• Citações Web of Science: 35

Resumo

There is a growing demand for natural colorants. This is prompting the search for new alternative and ``benign{''} separation systems allowing higher recoveries, extraction yields, and selectivities. This work investigates the use of aqueous two-phase systems (ATPS) based on ionic liquids as extraction processes for the recovery of red colorants from the fermented broth of Penicillium purpurogenum DPUA 1275. Several ATPS based on quaternary ammonium and imidazolium were studied in this work aiming at separating the red colorants produced from the remaining colorants and contaminant proteins present in the fermented broth. The results suggest that the red colorants can be isolated by an appropriate manipulation of some of the process conditions, such as the use of quaternary ammonium with short alkyl chains, alkaline media, and short tie-line lengths (extraction point systems with lower concentrations of ionic liquid). These conditions allow large partition coefficients for the red colorants (K (red) = 24.4 +/- A 2.3), high protein removal (60.7 +/- A 2.8 %) and selectivity parameters (S (red/prot) = 10.05). (AU)

• Processo FAPESP: 12/09479-4 - Recuperação a partir de meio fermentado de um colorante natural de aplicação industrial por sistemas aquosos bifásicos com líquidos iônicos
• Beneficiário: Adalberto Pessoa Junior
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisador Visitante - Internacional