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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Isolation of natural red colorants from fermented broth using ionic liquid-based aqueous two-phase systems

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Author(s):
Ventura, Sonia P. M. [1] ; Santos-Ebinuma, Valeria C. [2] ; Pereira, Jorge F. B. [1] ; Teixeira, Maria F. S. [3] ; Pessoa, Adalberto [2] ; Coutinho, Joao A. P. [1]
Total Authors: 6
Affiliation:
[1] Univ Aveiro, Dept Quim, CICECO, P-3810193 Aveiro - Portugal
[2] Univ Sao Paulo, Dept Tecnol Bioquim Farmaceut, BR-05508000 Sao Paulo - Brazil
[3] Univ Fed Amazonas, BR-69077000 Manaus, Amazonas - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Journal of Industrial Microbiology & Biotechnology; v. 40, n. 5, p. 507-516, MAY 2013.
Web of Science Citations: 35
Abstract

There is a growing demand for natural colorants. This is prompting the search for new alternative and ``benign{''} separation systems allowing higher recoveries, extraction yields, and selectivities. This work investigates the use of aqueous two-phase systems (ATPS) based on ionic liquids as extraction processes for the recovery of red colorants from the fermented broth of Penicillium purpurogenum DPUA 1275. Several ATPS based on quaternary ammonium and imidazolium were studied in this work aiming at separating the red colorants produced from the remaining colorants and contaminant proteins present in the fermented broth. The results suggest that the red colorants can be isolated by an appropriate manipulation of some of the process conditions, such as the use of quaternary ammonium with short alkyl chains, alkaline media, and short tie-line lengths (extraction point systems with lower concentrations of ionic liquid). These conditions allow large partition coefficients for the red colorants (K (red) = 24.4 +/- A 2.3), high protein removal (60.7 +/- A 2.8 %) and selectivity parameters (S (red/prot) = 10.05). (AU)

FAPESP's process: 12/09479-4 - Recovery of a natural colorant of industrial application from the fermentation broth using aqueous biphasic systems with ionic liquids
Grantee:Adalberto Pessoa Junior
Support type: Research Grants - Visiting Researcher Grant - International