Transcriptome meta-analysis of peripheral lymphomononuclear cells indicates that gestational diabetes is closer to type 1 diabetes than to type 2 diabetes mellitus

Autor(es):Mostrar menos -

Collares, C. V. A. ^{[1, 2]} ; Evangelista, A. F. ^[2] ; Xavier, D. J. ^[2] ; Takahashi, P. ^[2] ; Almeida, R. ^[2] ; Macedo, C. ^[2] ; Manoel-Caetano, F. ^[2] ; Foss, M. C. ^[3] ; Foss-Freitas, M. C. ^[3] ; Rassi, D. M. ^[1] ; Sakamoto-Hojo, E. T. ^{[2, 4]} ; Passos, G. A. ^[2] ; Donadi, E. A. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Med, Div Clin Immunol, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Mol Immunogenet Grp, BR-14049900 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Med, Div Endocrinol, BR-14049900 Ribeirao Preto, SP - Brazil

^[4] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, BR-14049 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MOLECULAR BIOLOGY REPORTS; v. 40, n. 9, p. 5351-5358, SEP 2013.
• Citações Web of Science: 10

Resumo

We performed a meta-analysis of the transcription profiles of type 1, type 2 and gestational diabetes to evaluate similarities and dissimilarities among these diabetes types. cRNA samples obtained from peripheral blood lymphomononuclear cells (PBMC) of 56 diabetes mellitus patients (type 1 = 19; type 2 = 20; gestational = 17) were hybridized to the same whole human genome oligomicroarray platform, encompassing 44,000 transcripts. The GeneSpring software was used to perform analysis and hierarchical clustering, and the DAVID database was used for gene ontology. The gene expression profiles showed more similarity between gestational and type 1 diabetes rather than between type 2 and gestational diabetes, a finding that was not influenced by patient gender and age. The meta-analysis of the three types of diabetes disclosed 3,747 differentially and significantly expressed genes. A total of 486 genes were characteristic of gestational diabetes, 202 genes of type 1, and 651 genes of type 2 diabetes. 19 known genes were shared by type 1, type 2 and gestational diabetes, highlighting EGF, FAM46C, HBEGF, ID1, SH3BGRL2, VEPH1, and TMEM158 genes. The meta-analysis of PBMC transcription profiles characterized each type of diabetes revealing that gestational and type 1 diabetes were transcriptionally related. (AU)

• Processo FAPESP: 10/05622-1 - Meta-análise do perfil de expressão gênica diferencial em pacientes com Diabetes Mellitus (DM-1, DM-2 e DMG)
• Beneficiário: Cristhianna Viesti Advincula Collares
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 10/12069-7 - Perfis de expressão gênica e possíveis interações entre microRNAs e mRNAs em Diabetes Mellitus tipo 1 com enfoque em resposta ao estresse oxidativo e reparo do DNA
• Beneficiário: Paula Takahashi
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 10/00932-2 - Perfis de expressão gênica associados ao processamento de dano oxidativo em pacientes com Diabetes Mellitus
• Beneficiário: Fernanda da Silva Manoel Caetano
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 08/56594-8 - Controle do transcriptoma no diabetes mellitus
• Beneficiário: Geraldo Aleixo da Silva Passos Júnior
• Modalidade de apoio: Auxílio à Pesquisa - Temático