Isobaric Tagging-Based Quantification for Proteomic Analysis: A Comparative Study of Spared and Affected Muscles from mdx Mice at the Early Phase of Dystrophy

Autor(es)

Matsumura, Cintia Yuri ^[1] ; de Oliveira, Bruno Menezes ^[2] ; Durbeej, Madeleine ^[2] ; Marques, Maria Julia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Inst Biol, Dept Biol Estrutural & Func, Sao Paulo - Brazil

^[2] Lund Univ, Dept Expt Med Sci, Muscle Biol Unit, Lund - Sweden

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 8, n. 6 JUN 18 2013.
• Citações Web of Science: 23

Resumo

Duchenne muscular dystrophy (DMD) is the most common childhood myopathy, characterized by muscle loss and cardiorespiratory failure. While the genetic basis of DMD is well established, secondary mechanisms associated with dystrophic pathophysiology are not fully clarified yet. In order to obtain new insights into the molecular mechanisms of muscle dystrophy during earlier stages of the disease, we performed a comparative proteomic profile of the spared extraocular muscles (EOM) vs. affected diaphragm from the mdx mice, using a label based shotgun proteomic approach. Out of the 857 identified proteins, 42 to 62 proteins had differential abundance of peptide ions. The calcium-handling proteins sarcalumenin and calsequestrin-1 were increased in control EOM compared with control DIA, reinforcing the view that constitutional properties of EOM are important for their protection against myonecrosis. The finding that galectin-1 (muscle regeneration), annexin A1 (anti-inflammatory) and HSP 47 (fibrosis) were increased in dystrophic diaphragm provides novel insights into the mechanisms through which mdx affected muscles are able to counteract dystrophy, during the early stage of the disease. Overall, the shotgun technique proved to be suitable to perform quantitative comparisons between distinct dystrophic muscles and allowed the suggestion of new potential biomarkers and drug targets for dystrophinopaties. (AU)

• Processo FAPESP: 11/51697-6 - Mecanismos de ação do ácido eicosapentanóico (EPA) na proteção a mionecrose em fibras musculares distróficas
• Beneficiário: Maria Julia Marques
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/58491-1 - Mecanismos de proteção da distrofia muscular: estudo proteômico e terapia farmacológica
• Beneficiário: Maria Julia Marques
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/54775-5 - Mecanismos de proteção da distrofia muscular: estudo proteômico e terapia farmacológica
• Beneficiário: Cintia Yuri Matsumura
• Linha de fomento: Bolsas no Brasil - Doutorado