Glucose transporter 1 expression accompanies hypoxia sensing in the cyclic canine corpus luteum

Autor(es)

Papa, Paula de Carvalho ^[1] ; Medeiros de Carvalho Sousa, Liza Margareth ^[1] ; Silva, Renata dos Santos ^[1] ; de Fatima, Luciana Alves ^[1] ; da Fonseca, Vanessa Uemura ^[1] ; do Amaral, Vanessa Coutinho ^[1] ; Hoffmann, Bernd ^[2] ; Alves-Wagner, Ana Barbara ^[3] ; Machado, Ubiratan Fabres ^[3] ; Kowalewski, Mariusz Pawel ^[4]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Surg, Sect Anat, BR-05508270 Sao Paulo - Brazil

^[2] Univ Giessen, Clin Obstet Gynecol & Androl Large & Small Anim, D-35390 Giessen - Germany

^[3] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, BR-05508270 Sao Paulo - Brazil

^[4] Univ Zurich, Inst Vet Anat, Zurich - Switzerland

• Tipo de documento: Artigo Científico
• Fonte: Reproduction; v. 147, n. 1, p. 81-89, JAN 2014.
• Citações Web of Science: 7

Resumo

The canine corpus luteum (CL) functions as a source of progesterone (P-4) and 17 beta-oestradiol (E-2); however, the transport of energy substrates to maintain its high hormonal output has not yet been characterised. This study involved the localisation and temporal distribution of the facilitative glucose transporter 1 and the quantification of the corresponding protein (GLUT1) and gene (SLC2A1) expression. Some GLUT1/SLC2A1 regulatory proteins, such as hypoxia-inducible factor 1 alpha (HIF1A) and fibroblast growth factor 2 (FGF2); mRNAs, such as HIF1A, FGF2 and vascular endothelial growth factor A (VEGFA); and VEGFA receptors 1 and 2 (FLT1 and KDR) were also analysed from days 10 to 70 after ovulation. Additionally, plasma P-4 and E-2 levels were assessed via chemiluminescence. Moreover, the canine KDR sequence has been cloned, thereby enabling subsequent semi-quantitative PCR analysis. Our results demonstrate time-dependent variations in the expression profile of SLC2A1 during dioestrus, which were accompanied by highly correlated changes (0.84<r<0.98; P<0.03) in the gene expression of HIF1A, VEGF and FLT1 as well as in P-4 plasma concentrations. FGF2 mRNA correlated with E-2 plasma concentrations (r=0.61; P=0.01). Our data reveal that the glucose transporter is regulated throughout the CL lifespan and suggest that CL depends on the sensing of hypoxia and the status of luteal vascularisation. Moreover, time-dependent expression of GLUT1/SLC2A1 may lie underneath increased metabolic and energetic requirements for sustaining P-4 production. (AU)

• Processo FAPESP: 10/07373-9 - Corpo lúteo e diestro na espécie canina: modelo de estudo para alterações metabólicas hormônio-dependentes
• Beneficiário: Paula de Carvalho Papa Keohane
• Modalidade de apoio: Bolsas no Exterior - Pesquisa
• Processo FAPESP: 08/54835-8 - Hipoxia e luteolise em cadelas nao prenhes
• Beneficiário: Liza Margareth Medeiros de Carvalho Sousa
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/17768-3 - O papel do 17b-estradiol no processo luteolítico de cadelas não prenhes
• Beneficiário: Paula de Carvalho Papa Keohane
• Modalidade de apoio: Auxílio à Pesquisa - Regular